Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9181 | 3' | -49.2 | NC_002512.2 | + | 103481 | 0.66 | 0.999828 |
Target: 5'- -gGUcuCgGCGGCg--GCCGcgGACCg -3' miRNA: 3'- caCAauGgUGCCGauaCGGCuaUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 222437 | 0.66 | 0.999828 |
Target: 5'- ----cGCCGCGGCUucccccggGCCGcg-GCCa -3' miRNA: 3'- cacaaUGGUGCCGAua------CGGCuauUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 224494 | 0.66 | 0.999824 |
Target: 5'- ----cGCCAaacacucCGGCUGUGUCGGgugcGCCa -3' miRNA: 3'- cacaaUGGU-------GCCGAUACGGCUau--UGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 219574 | 0.66 | 0.99978 |
Target: 5'- cGUG-UGCgACGGCUccuacGUcCUGGUGGCCg -3' miRNA: 3'- -CACaAUGgUGCCGA-----UAcGGCUAUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 151359 | 0.66 | 0.99978 |
Target: 5'- aUGUUGCCucaagAUGGCgccgGCCGAaAACg -3' miRNA: 3'- cACAAUGG-----UGCCGaua-CGGCUaUUGg -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 227673 | 0.66 | 0.99978 |
Target: 5'- ----aGCgGCGGCgccGCCGGcGACCg -3' miRNA: 3'- cacaaUGgUGCCGauaCGGCUaUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 104747 | 0.66 | 0.99978 |
Target: 5'- -cGggGCCcccGCGGUUcacgcGUGCUGcAUGACCa -3' miRNA: 3'- caCaaUGG---UGCCGA-----UACGGC-UAUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 217056 | 0.66 | 0.999722 |
Target: 5'- ----gGCCGCGuGCUgcAUGUCGGUGAUg -3' miRNA: 3'- cacaaUGGUGC-CGA--UACGGCUAUUGg -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 190012 | 0.66 | 0.999722 |
Target: 5'- -aGUUgGCCGCGGUcgagGCCGAcaUGAUCc -3' miRNA: 3'- caCAA-UGGUGCCGaua-CGGCU--AUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 96214 | 0.66 | 0.999722 |
Target: 5'- -gGUcgGCCACGGCg--GCCGcgGcggcggcggcgACCg -3' miRNA: 3'- caCAa-UGGUGCCGauaCGGCuaU-----------UGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 194618 | 0.66 | 0.99965 |
Target: 5'- ----cGCCGCGGCcGUcGCCGcggggGACCu -3' miRNA: 3'- cacaaUGGUGCCGaUA-CGGCua---UUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 184995 | 0.66 | 0.99965 |
Target: 5'- cGUGgagcGCUGCGGCccgGUGCCGcuGUAcagcGCCg -3' miRNA: 3'- -CACaa--UGGUGCCGa--UACGGC--UAU----UGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 225779 | 0.67 | 0.999458 |
Target: 5'- cUGUUcCCGaGGCUGcGCCGGccgGGCCg -3' miRNA: 3'- cACAAuGGUgCCGAUaCGGCUa--UUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 116736 | 0.67 | 0.999458 |
Target: 5'- -cGUgGCC-CGGCUgAUGgCGAUgGACCa -3' miRNA: 3'- caCAaUGGuGCCGA-UACgGCUA-UUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 228577 | 0.67 | 0.999331 |
Target: 5'- --cUUACCGCGGUcGUGUCGGcGGCa -3' miRNA: 3'- cacAAUGGUGCCGaUACGGCUaUUGg -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 203116 | 0.67 | 0.999331 |
Target: 5'- ----gGCCAgGGCgUcgGCCGAgucgugGGCCa -3' miRNA: 3'- cacaaUGGUgCCG-AuaCGGCUa-----UUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 90672 | 0.67 | 0.999331 |
Target: 5'- -cGgcgGCCGCGGCg--GCCag-GGCCa -3' miRNA: 3'- caCaa-UGGUGCCGauaCGGcuaUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 158660 | 0.67 | 0.999179 |
Target: 5'- -cGUcgagGCCACGGUcAUcGCCGGaucaUGGCCg -3' miRNA: 3'- caCAa---UGGUGCCGaUA-CGGCU----AUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 102557 | 0.67 | 0.998784 |
Target: 5'- -cGU--CCGCGGC---GUCGAUGGCCg -3' miRNA: 3'- caCAauGGUGCCGauaCGGCUAUUGG- -5' |
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9181 | 3' | -49.2 | NC_002512.2 | + | 124321 | 0.68 | 0.998238 |
Target: 5'- -cGggGCCGCGGCggacGUGCUGcgGcuguACCu -3' miRNA: 3'- caCaaUGGUGCCGa---UACGGCuaU----UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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