Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9182 | 3' | -55.4 | NC_002512.2 | + | 207565 | 0.66 | 0.973059 |
Target: 5'- -cGUCCCUCGcGUCC-CucgcGCUCCc -3' miRNA: 3'- ucCAGGGAGCcCAGGuGuaaaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 198513 | 0.66 | 0.973059 |
Target: 5'- cGGUCCgCUCGGGUCgC-CuucgauCUCCa -3' miRNA: 3'- uCCAGG-GAGCCCAG-GuGuaaac-GAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 184855 | 0.66 | 0.973059 |
Target: 5'- ---aUCCUCGGGUCCACcgUcagcgugcgcUGCUacugCCa -3' miRNA: 3'- uccaGGGAGCCCAGGUGuaA----------ACGA----GG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 176747 | 0.66 | 0.970325 |
Target: 5'- cAGGUCCUUCaGGGUCgcuucggacgccUGCAgg-GCUCg -3' miRNA: 3'- -UCCAGGGAG-CCCAG------------GUGUaaaCGAGg -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 113717 | 0.66 | 0.970325 |
Target: 5'- uGGGUCCCgacgCGGG-CgGCGaucUGCgggCCg -3' miRNA: 3'- -UCCAGGGa---GCCCaGgUGUaa-ACGa--GG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 218982 | 0.66 | 0.964272 |
Target: 5'- gAGGUCCggCccgGGGUCCGCAaccGcCUCCu -3' miRNA: 3'- -UCCAGGgaG---CCCAGGUGUaaaC-GAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 104748 | 0.66 | 0.960943 |
Target: 5'- gGGGcCCC-CGcGGUUCACGcgUGCUgCa -3' miRNA: 3'- -UCCaGGGaGC-CCAGGUGUaaACGAgG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 192962 | 0.66 | 0.957404 |
Target: 5'- -cGUCCCcCGGGUCCuccc--GCUCUg -3' miRNA: 3'- ucCAGGGaGCCCAGGuguaaaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 153059 | 0.67 | 0.953653 |
Target: 5'- cGGUCCCcggUCGGGUCgGCGgucgagGCg-- -3' miRNA: 3'- uCCAGGG---AGCCCAGgUGUaaa---CGagg -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 76934 | 0.67 | 0.949685 |
Target: 5'- gAGGaCCCcgcUCGcGGaUCCACAgucGCUCCc -3' miRNA: 3'- -UCCaGGG---AGC-CC-AGGUGUaaaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 60616 | 0.67 | 0.945497 |
Target: 5'- cAGGUCCCUC----CCACAcuugGCUCCc -3' miRNA: 3'- -UCCAGGGAGcccaGGUGUaaa-CGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 60686 | 0.67 | 0.945497 |
Target: 5'- cAGGUCCCUC----CCACAcuugGCUCCc -3' miRNA: 3'- -UCCAGGGAGcccaGGUGUaaa-CGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 87789 | 0.67 | 0.936452 |
Target: 5'- cGGGUCgguaC-CGGGUCCGCGUucggUUGCaCCc -3' miRNA: 3'- -UCCAGg---GaGCCCAGGUGUA----AACGaGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 204807 | 0.69 | 0.897678 |
Target: 5'- gAGGUCCgCguccCGGGggCGCGgcgGCUCCg -3' miRNA: 3'- -UCCAGG-Ga---GCCCagGUGUaaaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 169631 | 0.7 | 0.855927 |
Target: 5'- cAGGUCCaggugUCGGGUCUGCGgcaUGaUUCCg -3' miRNA: 3'- -UCCAGGg----AGCCCAGGUGUaa-AC-GAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 153009 | 0.7 | 0.83239 |
Target: 5'- cGGUCCCcggUCGGGUCgGCGggaGCggUCCc -3' miRNA: 3'- uCCAGGG---AGCCCAGgUGUaaaCG--AGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 152959 | 0.7 | 0.83239 |
Target: 5'- cGGUCCCcggUCGGGUCgGCGggaGCggUCCc -3' miRNA: 3'- uCCAGGG---AGCCCAGgUGUaaaCG--AGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 218120 | 0.7 | 0.824189 |
Target: 5'- gGGGUCgCUCGGGUagaaCACGUcgucgucgucgUcGCUCCc -3' miRNA: 3'- -UCCAGgGAGCCCAg---GUGUA-----------AaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 88482 | 0.71 | 0.798623 |
Target: 5'- cAGGaUCUUCGGGuggaUCCGCAgccGCUCCg -3' miRNA: 3'- -UCCaGGGAGCCC----AGGUGUaaaCGAGG- -5' |
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9182 | 3' | -55.4 | NC_002512.2 | + | 218297 | 0.71 | 0.780851 |
Target: 5'- gGGGUCCCcggcccUGGGcggCCGCucgUGCUCCc -3' miRNA: 3'- -UCCAGGGa-----GCCCa--GGUGuaaACGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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