Results 1 - 20 of 58 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9193 | 5' | -61.5 | NC_002512.2 | + | 1600 | 0.71 | 0.498343 |
Target: 5'- cCGGGUCCCGUCCucccucucccacccGgUCCGcGGUCaGUc -3' miRNA: 3'- -GCCCAGGGCAGG--------------UgAGGCuCCAGcCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 1885 | 0.66 | 0.786447 |
Target: 5'- cCGcGUCCCGUCCgGCUCCGGcccUCGGc -3' miRNA: 3'- -GCcCAGGGCAGG-UGAGGCUcc-AGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 5482 | 0.67 | 0.758723 |
Target: 5'- uCGGGgaccucguggcgCgCGUCCGCgCCGGGGUCGcGa -3' miRNA: 3'- -GCCCa-----------GgGCAGGUGaGGCUCCAGC-Ca -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 5771 | 0.67 | 0.733652 |
Target: 5'- --uGUCCCGUCCGCUCaGAcGGUuCGGc -3' miRNA: 3'- gccCAGGGCAGGUGAGgCU-CCA-GCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 6562 | 0.67 | 0.75163 |
Target: 5'- aGGGUCCCGUCCuC-CCGAccGUucaCGGUg -3' miRNA: 3'- gCCCAGGGCAGGuGaGGCUc-CA---GCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 8701 | 1.06 | 0.002888 |
Target: 5'- uCGGGUCCCGUCCACUCCGAGGUCGGUc -3' miRNA: 3'- -GCCCAGGGCAGGUGAGGCUCCAGCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 17383 | 0.78 | 0.205352 |
Target: 5'- uCGuGGUCCgCGUCCAgcCCGAGGUCGGa -3' miRNA: 3'- -GC-CCAGG-GCAGGUgaGGCUCCAGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 19672 | 0.68 | 0.696851 |
Target: 5'- uCGGGUCCgCGUCUccguCUCgGAcgaucagacGGUCGGg -3' miRNA: 3'- -GCCCAGG-GCAGGu---GAGgCU---------CCAGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 28535 | 0.66 | 0.786447 |
Target: 5'- uCGGGUCcgCCG-CCGC-CCGAuGUCGGc -3' miRNA: 3'- -GCCCAG--GGCaGGUGaGGCUcCAGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 32010 | 0.7 | 0.555708 |
Target: 5'- cCGGGUCCCGUCacuuGCcCCGcaGUCGGUu -3' miRNA: 3'- -GCCCAGGGCAGg---UGaGGCucCAGCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 32083 | 0.66 | 0.814567 |
Target: 5'- gGGGUCgCCGauggcggcccgggugUCCucGCggUCGAGGUCGGg -3' miRNA: 3'- gCCCAG-GGC---------------AGG--UGa-GGCUCCAGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 37309 | 0.69 | 0.621458 |
Target: 5'- gCGGGa-CgGUCCGCUCCGAGGcccCGGc -3' miRNA: 3'- -GCCCagGgCAGGUGAGGCUCCa--GCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 83127 | 0.67 | 0.724547 |
Target: 5'- uCGGGUCgUCGUCCcCgucgUCGAGGUCGu- -3' miRNA: 3'- -GCCCAG-GGCAGGuGa---GGCUCCAGCca -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 84679 | 0.66 | 0.777903 |
Target: 5'- -uGGUCCuCGUCCcgcaGC-CCGGGGUCGa- -3' miRNA: 3'- gcCCAGG-GCAGG----UGaGGCUCCAGCca -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 93457 | 0.68 | 0.686581 |
Target: 5'- cCGGGUCCCcucguGUCCGgUCaugggauaccagaUGGGGUUGGUu -3' miRNA: 3'- -GCCCAGGG-----CAGGUgAG-------------GCUCCAGCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 93854 | 0.68 | 0.706139 |
Target: 5'- aCGGGUCgCGgucUCCcUUCCGAGGUucucaCGGUu -3' miRNA: 3'- -GCCCAGgGC---AGGuGAGGCUCCA-----GCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 94021 | 0.66 | 0.819366 |
Target: 5'- aGGGcCUCGUCCgccaGCUCCucGG-CGGUg -3' miRNA: 3'- gCCCaGGGCAGG----UGAGGcuCCaGCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 102040 | 0.66 | 0.794872 |
Target: 5'- aCGGGaUCCCGcUCCggACUCCGGaG-CGGUu -3' miRNA: 3'- -GCCC-AGGGC-AGG--UGAGGCUcCaGCCA- -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 111926 | 0.67 | 0.724547 |
Target: 5'- uCGGGUgCCGgaacaGCUCC-AGGUCGGc -3' miRNA: 3'- -GCCCAgGGCagg--UGAGGcUCCAGCCa -5' |
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9193 | 5' | -61.5 | NC_002512.2 | + | 113186 | 0.71 | 0.5281 |
Target: 5'- cCGGcGUCgCCGUCCGCUCCGcgagcGGGagcCGGg -3' miRNA: 3'- -GCC-CAG-GGCAGGUGAGGC-----UCCa--GCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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