Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 92 | 3' | -59.6 | AC_000006.1 | + | 7477 | 0.67 | 0.312825 |
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Target: 5'- gAAGGaaGCccaGAGCUCgCGgGCCAugAGGGUCu -3' miRNA: 3'- gUUCC--CG---UUCGAG-GCgCGGU--UCCCGG- -5' |
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| 92 | 3' | -59.6 | AC_000006.1 | + | 20401 | 0.66 | 0.318331 |
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Target: 5'- ---cGGCAGcgaccaguagacuuGCUCCGCGCCGcaggugacguccGGGGUg -3' miRNA: 3'- guucCCGUU--------------CGAGGCGCGGU------------UCCCGg -5' |
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| 92 | 3' | -59.6 | AC_000006.1 | + | 1896 | 0.66 | 0.328748 |
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Target: 5'- gCAGGGGCuacauucuGGaCUUCGCaGCCAugcaccuguggAGGGCa -3' miRNA: 3'- -GUUCCCGu-------UC-GAGGCG-CGGU-----------UCCCGg -5' |
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| 92 | 3' | -59.6 | AC_000006.1 | + | 13027 | 0.66 | 0.353738 |
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Target: 5'- cCAGGaGGCGGuGCgcacgcCCGCGagCGAGGGCg -3' miRNA: 3'- -GUUC-CCGUU-CGa-----GGCGCg-GUUCCCGg -5' |
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| 92 | 3' | -59.6 | AC_000006.1 | + | 22635 | 0.66 | 0.36236 |
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Target: 5'- gAGGGGCuccucucguGCUCCggcggauagcGCGCCGacccguggccccGGGGCg -3' miRNA: 3'- gUUCCCGuu-------CGAGG----------CGCGGU------------UCCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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