Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9283 | 3' | -50.7 | NC_002512.2 | + | 224613 | 0.66 | 0.998785 |
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Target: 5'- cGGUGCCgACCGGgcucgGGGUcgCCg---- -3' miRNA: 3'- uCCAUGGaUGGCCa----UCCAa-GGauauc -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 41548 | 0.66 | 0.997873 |
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Target: 5'- gAGGUugcggGCCUGCaCGGUggcgcgcAGGUUCUUGa-- -3' miRNA: 3'- -UCCA-----UGGAUG-GCCA-------UCCAAGGAUauc -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 110571 | 0.68 | 0.990518 |
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Target: 5'- gAGGU-CCUGCCGGgucAGGaucUCCUGa-- -3' miRNA: 3'- -UCCAuGGAUGGCCa--UCCa--AGGAUauc -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 154197 | 0.71 | 0.967273 |
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Target: 5'- uAGGUGCCcguaggACCuaGGaUAGGUcCCUAUAGg -3' miRNA: 3'- -UCCAUGGa-----UGG--CC-AUCCAaGGAUAUC- -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 158548 | 0.72 | 0.929724 |
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Target: 5'- uGGUggACCUGCUGGUGGGccgcUCCUGg-- -3' miRNA: 3'- uCCA--UGGAUGGCCAUCCa---AGGAUauc -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 153638 | 0.75 | 0.841601 |
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Target: 5'- uAGG-ACCUACCGGUAGGa-CCUAc-- -3' miRNA: 3'- -UCCaUGGAUGGCCAUCCaaGGAUauc -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 76251 | 0.82 | 0.478663 |
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Target: 5'- uAGGU-CCUACCGGUAGG-UCCUaccgGUAGg -3' miRNA: 3'- -UCCAuGGAUGGCCAUCCaAGGA----UAUC- -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 153664 | 0.91 | 0.17677 |
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Target: 5'- uAGGaACCUACCGGUAGGUaCCUAUAGc -3' miRNA: 3'- -UCCaUGGAUGGCCAUCCAaGGAUAUC- -5' |
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| 9283 | 3' | -50.7 | NC_002512.2 | + | 76223 | 1.1 | 0.011966 |
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Target: 5'- uAGGUACCUACCGGUAGGUUCCUAUAGg -3' miRNA: 3'- -UCCAUGGAUGGCCAUCCAAGGAUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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