Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9287 | 5' | -58.3 | NC_002512.2 | + | 149170 | 0.66 | 0.893724 |
Target: 5'- gCCGCCCgc-GGGCccgcgcgcccucgCCCCCCGGAc-- -3' miRNA: 3'- -GGUGGGacaCCCGa------------GGGGGGUUUaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 16530 | 0.66 | 0.889871 |
Target: 5'- gCCGCCagcGcGGGCgUCCCCCUggcuccGGAUGUa -3' miRNA: 3'- -GGUGGga-CaCCCG-AGGGGGG------UUUACA- -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 69142 | 0.66 | 0.896251 |
Target: 5'- -uGCCCg--GGGCcggcgccguUCCCCCCGAAa-- -3' miRNA: 3'- ggUGGGacaCCCG---------AGGGGGGUUUaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 22127 | 0.66 | 0.902419 |
Target: 5'- aCCACCacgGcgGcGGCUCCaCCCCGGAg-- -3' miRNA: 3'- -GGUGGga-Ca-C-CCGAGG-GGGGUUUaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 44352 | 0.67 | 0.862304 |
Target: 5'- uUCGCCCUGUGGuGCgCCCUgU--GUGUg -3' miRNA: 3'- -GGUGGGACACC-CGaGGGGgGuuUACA- -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 133370 | 0.67 | 0.839609 |
Target: 5'- aCCGCCg-GcGcGGCUCCCUCCGGcgGUc -3' miRNA: 3'- -GGUGGgaCaC-CCGAGGGGGGUUuaCA- -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 137202 | 0.67 | 0.850404 |
Target: 5'- uCCGCCCgGUGGGCggcgcgCCCggcgcgggcggccguCCCGGAUc- -3' miRNA: 3'- -GGUGGGaCACCCGa-----GGG---------------GGGUUUAca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 10045 | 0.67 | 0.854923 |
Target: 5'- uCCGCCCcc-GGGCcggaCCCCCCGAccGa -3' miRNA: 3'- -GGUGGGacaCCCGa---GGGGGGUUuaCa -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 201828 | 0.67 | 0.847356 |
Target: 5'- gCGCCCgg-GGGCUCCUUCCGc---- -3' miRNA: 3'- gGUGGGacaCCCGAGGGGGGUuuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 155702 | 0.67 | 0.854923 |
Target: 5'- gCCGCCCUGUGccGGCg-CCUCCAccUGg -3' miRNA: 3'- -GGUGGGACAC--CCGagGGGGGUuuACa -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 177680 | 0.68 | 0.794947 |
Target: 5'- gCCGCCaguuugcgguagaUGgggaucagGGGCUUCCCCCAGGUcGUg -3' miRNA: 3'- -GGUGGg------------ACa-------CCCGAGGGGGGUUUA-CA- -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 224705 | 0.68 | 0.806952 |
Target: 5'- cCCGCCCUGcugGGGCcggccgCCgCCCCGGu--- -3' miRNA: 3'- -GGUGGGACa--CCCGa-----GG-GGGGUUuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 45729 | 0.68 | 0.8236 |
Target: 5'- aCCGCCCUacgccaUGGcGCUaCCCCCCAu---- -3' miRNA: 3'- -GGUGGGAc-----ACC-CGA-GGGGGGUuuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 21855 | 0.68 | 0.798405 |
Target: 5'- aCCACCCgGgccaGGGuCUCCgCCCCGAc--- -3' miRNA: 3'- -GGUGGGaCa---CCC-GAGG-GGGGUUuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 121498 | 0.69 | 0.753751 |
Target: 5'- cCCACCCaccGGGCUCgCCCCCc----- -3' miRNA: 3'- -GGUGGGacaCCCGAG-GGGGGuuuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 118699 | 0.69 | 0.762911 |
Target: 5'- gCGCC--GUGGGCUUCCCCCGc---- -3' miRNA: 3'- gGUGGgaCACCCGAGGGGGGUuuaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 105894 | 0.7 | 0.716205 |
Target: 5'- cCCACCCgGUGGGCUCgCCgUCGuc-GUa -3' miRNA: 3'- -GGUGGGaCACCCGAG-GGgGGUuuaCA- -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 24781 | 0.7 | 0.716205 |
Target: 5'- cCCGCUCggGUccGGGCgcgCCCCCCGAGg-- -3' miRNA: 3'- -GGUGGGa-CA--CCCGa--GGGGGGUUUaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 109395 | 0.72 | 0.589442 |
Target: 5'- aCCAUCCUG-GGGUUCCUggCCCAGAa-- -3' miRNA: 3'- -GGUGGGACaCCCGAGGG--GGGUUUaca -5' |
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9287 | 5' | -58.3 | NC_002512.2 | + | 120061 | 0.72 | 0.579701 |
Target: 5'- gCCGCCCcagGGGaCUCCCCUCAGAcUGa -3' miRNA: 3'- -GGUGGGacaCCC-GAGGGGGGUUU-ACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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