Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9353 | 3' | -48 | NC_002512.2 | + | 208893 | 0.66 | 0.99998 |
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Target: 5'- gGGGcCgg---GAAUCcCCGCG-CGGGa -3' miRNA: 3'- -CCCaGauauaUUUAGaGGCGCaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 176609 | 0.66 | 0.99998 |
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Target: 5'- uGG-CcGUGUcGAUCUCCacccCGUCGGGg -3' miRNA: 3'- cCCaGaUAUAuUUAGAGGc---GCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 155654 | 0.66 | 0.999982 |
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Target: 5'- cGGcGUCcggccgu-UCgccCCGCGUCGGGa -3' miRNA: 3'- -CC-CAGauauauuuAGa--GGCGCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 152970 | 0.66 | 0.999985 |
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Target: 5'- cGGGUCggcgggAgcGGUCcCCG-GUCGGGu -3' miRNA: 3'- -CCCAGaua---UauUUAGaGGCgCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 153020 | 0.66 | 0.999985 |
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Target: 5'- cGGGUCggcgggAgcGGUCcCCG-GUCGGGu -3' miRNA: 3'- -CCCAGaua---UauUUAGaGGCgCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 205869 | 0.66 | 0.999985 |
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Target: 5'- cGGUagUGUGUAuuauUUUUCGCGUCGcGGg -3' miRNA: 3'- cCCAg-AUAUAUuu--AGAGGCGCAGC-CC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 196358 | 0.66 | 0.999989 |
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Target: 5'- aGGGUCg----GAggCUCCGCcgGcCGGGc -3' miRNA: 3'- -CCCAGauauaUUuaGAGGCG--CaGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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