Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9353 | 3' | -48 | NC_002512.2 | + | 205126 | 0.67 | 0.999937 |
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Target: 5'- cGGGUCUuc----GUCUCCGa--CGGGa -3' miRNA: 3'- -CCCAGAuauauuUAGAGGCgcaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 205869 | 0.66 | 0.999985 |
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Target: 5'- cGGUagUGUGUAuuauUUUUCGCGUCGcGGg -3' miRNA: 3'- cCCAg-AUAUAUuu--AGAGGCGCAGC-CC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 208893 | 0.66 | 0.99998 |
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Target: 5'- gGGGcCgg---GAAUCcCCGCG-CGGGa -3' miRNA: 3'- -CCCaGauauaUUUAGaGGCGCaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 219037 | 0.69 | 0.999453 |
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Target: 5'- uGGUCUAcGUc-GUCggCCGCGgcgCGGGg -3' miRNA: 3'- cCCAGAUaUAuuUAGa-GGCGCa--GCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 220015 | 0.71 | 0.996154 |
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Target: 5'- cGGGUCgagaggccgCUCCGUcUCGGGg -3' miRNA: 3'- -CCCAGauauauuuaGAGGCGcAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 220164 | 0.7 | 0.998785 |
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Target: 5'- gGGcGUCgug------CUCUGCGUCGGGa -3' miRNA: 3'- -CC-CAGauauauuuaGAGGCGCAGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 224112 | 0.66 | 0.999973 |
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Target: 5'- cGGUCgugGUGAccAUCUcggccguggacCCGCGcCGGGa -3' miRNA: 3'- cCCAGauaUAUU--UAGA-----------GGCGCaGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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