Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9353 | 3' | -48 | NC_002512.2 | + | 111705 | 0.67 | 0.999893 |
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Target: 5'- gGGGUCgcggAUGacgacgGAGUCUCCG-G-CGGGc -3' miRNA: 3'- -CCCAGa---UAUa-----UUUAGAGGCgCaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 54445 | 0.66 | 0.999973 |
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Target: 5'- cGGGUCU----GAAUCaCCGaCG-CGGGa -3' miRNA: 3'- -CCCAGAuauaUUUAGaGGC-GCaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 54251 | 0.66 | 0.999973 |
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Target: 5'- cGGGUCU----GAAUCaCCGaCG-CGGGa -3' miRNA: 3'- -CCCAGAuauaUUUAGaGGC-GCaGCCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 38979 | 0.68 | 0.999824 |
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Target: 5'- cGGGUCUGUGUAAAag--CGUGUCGuuuGGa -3' miRNA: 3'- -CCCAGAUAUAUUUagagGCGCAGC---CC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 34550 | 0.66 | 0.999989 |
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Target: 5'- cGGGUcCUAgcu---UCgUCCGCGUCaGGu -3' miRNA: 3'- -CCCA-GAUauauuuAG-AGGCGCAGcCC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 33432 | 0.67 | 0.999916 |
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Target: 5'- uGGGUCgucGUGaucggagaacugcGAUCUCCG-GUCGuGGg -3' miRNA: 3'- -CCCAGauaUAU-------------UUAGAGGCgCAGC-CC- -5' |
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| 9353 | 3' | -48 | NC_002512.2 | + | 27024 | 0.68 | 0.999776 |
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Target: 5'- uGGUCUAUAaacGGAUCcaCCGCuGUCGGu -3' miRNA: 3'- cCCAGAUAUa--UUUAGa-GGCG-CAGCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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