Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9369 | 3' | -47.2 | NC_002512.2 | + | 217177 | 0.69 | 0.999724 |
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Target: 5'- gUCGggUUcaUCGCCCUCcgcGCCAgGUu -3' miRNA: 3'- -GGCuuAAa-AGCGGGAGuaaUGGUgCA- -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 204316 | 0.69 | 0.999653 |
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Target: 5'- uCCGcuGGUcgUCGCCCUCA--ACCGCc- -3' miRNA: 3'- -GGC--UUAaaAGCGGGAGUaaUGGUGca -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 208824 | 0.69 | 0.999566 |
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Target: 5'- gCCGcccgugCGCCCUCAcgcGCCGCGc -3' miRNA: 3'- -GGCuuaaaaGCGGGAGUaa-UGGUGCa -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 216856 | 0.7 | 0.998531 |
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Target: 5'- gUGAuc--UCGCCCUCGUcguCCGCGUc -3' miRNA: 3'- gGCUuaaaAGCGGGAGUAau-GGUGCA- -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 155659 | 0.72 | 0.994434 |
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Target: 5'- uCCGGccgUUCGCCCcgcgUCGggACCGCGg -3' miRNA: 3'- -GGCUuaaAAGCGGG----AGUaaUGGUGCa -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 171415 | 0.72 | 0.992576 |
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Target: 5'- gCCGggUc-UCGCUCUCcuGUUACCugGg -3' miRNA: 3'- -GGCuuAaaAGCGGGAG--UAAUGGugCa -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 218877 | 0.73 | 0.987398 |
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Target: 5'- aCCGAcgccGUgcgCGCCCUCAUcgcgcGCCACGc -3' miRNA: 3'- -GGCU----UAaaaGCGGGAGUAa----UGGUGCa -5' |
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| 9369 | 3' | -47.2 | NC_002512.2 | + | 219654 | 0.74 | 0.981956 |
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Target: 5'- aCCGGAggUUCGCCCgUCGggAUCugGg -3' miRNA: 3'- -GGCUUaaAAGCGGG-AGUaaUGGugCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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