Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9376 | 5' | -53.9 | NC_002512.2 | + | 78328 | 0.66 | 0.985254 |
Target: 5'- cGACCGucCGgACCGCGGaaGGUUCGu -3' miRNA: 3'- -CUGGCucGCaUGGUGCCagCUAAGCu -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 199575 | 0.66 | 0.983491 |
Target: 5'- aGACuCGuGCgGUACCGCGgGUCG--UCGAc -3' miRNA: 3'- -CUG-GCuCG-CAUGGUGC-CAGCuaAGCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 193889 | 0.66 | 0.983491 |
Target: 5'- cGACgcggGAGCGggucGCCGCGGUCGg--CGGa -3' miRNA: 3'- -CUGg---CUCGCa---UGGUGCCAGCuaaGCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 190004 | 0.67 | 0.981573 |
Target: 5'- cGACCucaAGUugGCCGCGGUCGAggcCGAc -3' miRNA: 3'- -CUGGc--UCGcaUGGUGCCAGCUaa-GCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 119561 | 0.7 | 0.901791 |
Target: 5'- gGACCGGGCGgACCGCGGgagcccggCGggUCc- -3' miRNA: 3'- -CUGGCUCGCaUGGUGCCa-------GCuaAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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