Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9376 | 5' | -53.9 | NC_002512.2 | + | 188936 | 0.66 | 0.98687 |
Target: 5'- uGGCgGGGC--GCgGCGGUCGGUUCc- -3' miRNA: 3'- -CUGgCUCGcaUGgUGCCAGCUAAGcu -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 105776 | 0.66 | 0.989689 |
Target: 5'- aACCuGGGCGU-CgCAUGGUCGAcggCGAg -3' miRNA: 3'- cUGG-CUCGCAuG-GUGCCAGCUaa-GCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 153397 | 0.66 | 0.989689 |
Target: 5'- cGACgugGAGCGcgGCgGCGGUCGAgguggCGGa -3' miRNA: 3'- -CUGg--CUCGCa-UGgUGCCAGCUaa---GCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 226689 | 0.66 | 0.989689 |
Target: 5'- cGCCGGGcCGUcgcCCGCGG-CGGcgUCGAg -3' miRNA: 3'- cUGGCUC-GCAu--GGUGCCaGCUa-AGCU- -5' |
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9376 | 5' | -53.9 | NC_002512.2 | + | 227429 | 0.66 | 0.989689 |
Target: 5'- cGCgCGGGCGcggGCCGCGGUCaccUUCGc -3' miRNA: 3'- cUG-GCUCGCa--UGGUGCCAGcu-AAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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