miRNA: 3'- cuGGCUCGcaUGGUGCCAGCUa-AGcu -5'
miRNA: 3'- cuGGCUCGCauggUGCCAGC---UAAGCU- -5'
miRNA: 3'- -CUGGCUCGCA---UGG---UGCCaGCuaAGCu -5'
miRNA: 3'- -CUGGCUC-GCaUGGUGCCAGCua--AGcu -5'
miRNA: 3'- -CUGGCUCGCaUGGUGCCa-------GCuaAGcu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9376 | 5' | -53.9 | NC_002512.2 | + | 189526 | 0.68 | 0.963218 |
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Target: 5'- -cCCGGGCucGCCGCGGUCGGcgUCc- -3' miRNA: 3'- cuGGCUCGcaUGGUGCCAGCUa-AGcu -5' |
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| 9376 | 5' | -53.9 | NC_002512.2 | + | 108592 | 0.68 | 0.959816 |
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Target: 5'- -uCCGGGCGgagaGCGGUCGcaaGUUCGAc -3' miRNA: 3'- cuGGCUCGCauggUGCCAGC---UAAGCU- -5' |
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| 9376 | 5' | -53.9 | NC_002512.2 | + | 87835 | 0.69 | 0.944064 |
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Target: 5'- cGGCCGGGCGUcggGCCcucGCGG-CGggUCGc -3' miRNA: 3'- -CUGGCUCGCA---UGG---UGCCaGCuaAGCu -5' |
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| 9376 | 5' | -53.9 | NC_002512.2 | + | 148067 | 0.69 | 0.929913 |
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Target: 5'- aGAUCGAGaCGcGCCGCGGUCGuccgUCc- -3' miRNA: 3'- -CUGGCUC-GCaUGGUGCCAGCua--AGcu -5' |
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| 9376 | 5' | -53.9 | NC_002512.2 | + | 119561 | 0.7 | 0.901791 |
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Target: 5'- gGACCGGGCGgACCGCGGgagcccggCGggUCc- -3' miRNA: 3'- -CUGGCUCGCaUGGUGCCa-------GCuaAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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