Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9381 | 3' | -55.1 | NC_002512.2 | + | 147261 | 0.7 | 0.855927 |
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Target: 5'- aGAGAaGUGGgaaaCCCGAcccgGgacGCCGCGCGUc -3' miRNA: 3'- -CUCUaUACCa---GGGCUa---C---UGGCGCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 166189 | 0.7 | 0.840417 |
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Target: 5'- gGAGAUccccGUGG-CCuucuugggcggCGAggagGGCCGCGCGCg -3' miRNA: 3'- -CUCUA----UACCaGG-----------GCUa---CUGGCGCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 224460 | 0.72 | 0.775418 |
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Target: 5'- ----cGUGGUCUCGGUGuuauuauaaauccagGCUGCGCGCc -3' miRNA: 3'- cucuaUACCAGGGCUAC---------------UGGCGCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 141278 | 0.72 | 0.753265 |
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Target: 5'- -------cGUCCCGAcgccGGCCGCGCGCa -3' miRNA: 3'- cucuauacCAGGGCUa---CUGGCGCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 174755 | 0.73 | 0.715105 |
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Target: 5'- aGGAUGUGGUCCag--GACCGCGUcCg -3' miRNA: 3'- cUCUAUACCAGGgcuaCUGGCGCGcG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 209345 | 0.73 | 0.714135 |
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Target: 5'- gGAGGUGUGGUacgaggucggcgaCCUGgcGACCGUGUGUc -3' miRNA: 3'- -CUCUAUACCA-------------GGGCuaCUGGCGCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 188924 | 0.73 | 0.705376 |
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Target: 5'- gGAGA----GUCCCGGUGGCgGgGCGCg -3' miRNA: 3'- -CUCUauacCAGGGCUACUGgCgCGCG- -5' |
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| 9381 | 3' | -55.1 | NC_002512.2 | + | 123632 | 0.74 | 0.685749 |
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Target: 5'- aAGAcg-GGUCCCGggGAgaGCGCGCc -3' miRNA: 3'- cUCUauaCCAGGGCuaCUggCGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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