Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9388 | 5' | -52.9 | NC_002512.2 | + | 147709 | 0.72 | 0.857369 |
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Target: 5'- -gCGGAcgGGCucuCGACCCucCUGACGCu -3' miRNA: 3'- caGCCUaaCUG---GCUGGGuuGACUGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 102871 | 0.72 | 0.854307 |
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Target: 5'- uGUCGGAgaaacgcgcccgGACCGACuCCAccgugcgcCUGGCGCg -3' miRNA: 3'- -CAGCCUaa----------CUGGCUG-GGUu-------GACUGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 213724 | 0.74 | 0.790684 |
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Target: 5'- cGUCGGAgcugacgacgUUGACCGACCgGGCgcagGcCGCc -3' miRNA: 3'- -CAGCCU----------AACUGGCUGGgUUGa---CuGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 172405 | 0.74 | 0.772466 |
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Target: 5'- -cCGGGaUGACCG-CCCGGCUGGaGCa -3' miRNA: 3'- caGCCUaACUGGCuGGGUUGACUgCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 225424 | 0.75 | 0.763171 |
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Target: 5'- gGUCGGGUUGggGCCGACgCCGACccccGCGCc -3' miRNA: 3'- -CAGCCUAAC--UGGCUG-GGUUGac--UGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 78252 | 0.75 | 0.734641 |
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Target: 5'- cUCGGAgacgcGAUCGGCCCucCUGACGUu -3' miRNA: 3'- cAGCCUaa---CUGGCUGGGuuGACUGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 192582 | 0.75 | 0.715174 |
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Target: 5'- cUCGGAgcgGACCGuCCCGcuGCUGcgGCGCg -3' miRNA: 3'- cAGCCUaa-CUGGCuGGGU--UGAC--UGCG- -5' |
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| 9388 | 5' | -52.9 | NC_002512.2 | + | 99415 | 0.76 | 0.695432 |
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Target: 5'- aUCGGGccccGACCGACCCAg--GACGCu -3' miRNA: 3'- cAGCCUaa--CUGGCUGGGUugaCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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