Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9388 | 5' | -52.9 | NC_002512.2 | + | 219838 | 0.71 | 0.899314 |
Target: 5'- gGUCGGGggGuCCGGCCCGgggGCgGAuCGCg -3' miRNA: 3'- -CAGCCUaaCuGGCUGGGU---UGaCU-GCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 220944 | 0.7 | 0.938055 |
Target: 5'- cGUCGGAacGcgcucuucaucGCCGACCCGGCcGAgGCc -3' miRNA: 3'- -CAGCCUaaC-----------UGGCUGGGUUGaCUgCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 221242 | 0.66 | 0.993809 |
Target: 5'- gGUC-GAUcGGCCGGCCgCGGCggaagucggggGACGCg -3' miRNA: 3'- -CAGcCUAaCUGGCUGG-GUUGa----------CUGCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 223188 | 0.69 | 0.958837 |
Target: 5'- -gUGGAcgGGCCG-CCCGACgggGACGg -3' miRNA: 3'- caGCCUaaCUGGCuGGGUUGa--CUGCg -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 224109 | 0.66 | 0.991879 |
Target: 5'- uGUCGGucguggUGACCaucucggccgugGACCCGcgccgggagGCgGACGCg -3' miRNA: 3'- -CAGCCua----ACUGG------------CUGGGU---------UGaCUGCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 224376 | 0.67 | 0.986611 |
Target: 5'- cUCGGuccggGACCucgcGAcCCCGGCgcgGACGCg -3' miRNA: 3'- cAGCCuaa--CUGG----CU-GGGUUGa--CUGCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 225424 | 0.75 | 0.763171 |
Target: 5'- gGUCGGGUUGggGCCGACgCCGACccccGCGCc -3' miRNA: 3'- -CAGCCUAAC--UGGCUG-GGUUGac--UGCG- -5' |
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9388 | 5' | -52.9 | NC_002512.2 | + | 225565 | 0.66 | 0.99075 |
Target: 5'- uUCGGcgacgaGGCCGACCuCGAggGGCGCc -3' miRNA: 3'- cAGCCuaa---CUGGCUGG-GUUgaCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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