Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9399 | 5' | -55.6 | NC_002513.1 | + | 32709 | 0.66 | 0.53012 |
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Target: 5'- --gUGaggCGCAGUGCUgaGCuaAAGCGCAc -3' miRNA: 3'- caaACa--GCGUCGCGG--UGcgUUCGCGU- -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 13992 | 0.66 | 0.506865 |
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Target: 5'- ---cGcCGCcgacgcagagcagAGCGCCGcCGCAGGCGUc -3' miRNA: 3'- caaaCaGCG-------------UCGCGGU-GCGUUCGCGu -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 10737 | 0.66 | 0.497031 |
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Target: 5'- ---gGUCGCgggacgugAGCGCCAUGau-GCGCu -3' miRNA: 3'- caaaCAGCG--------UCGCGGUGCguuCGCGu -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 16140 | 0.66 | 0.475496 |
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Target: 5'- ----uUCuaGGCGCCGCuGCAcAGCGCGa -3' miRNA: 3'- caaacAGcgUCGCGGUG-CGU-UCGCGU- -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 23345 | 0.67 | 0.444097 |
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Target: 5'- -cUUGUCGCGGCgGCUA-GUuuuuuuGAGCGCGg -3' miRNA: 3'- caAACAGCGUCG-CGGUgCG------UUCGCGU- -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 12095 | 0.7 | 0.29873 |
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Target: 5'- --aUGUCGCgcuGGCG-CACGUAcGCGCAa -3' miRNA: 3'- caaACAGCG---UCGCgGUGCGUuCGCGU- -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 16026 | 0.74 | 0.149188 |
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Target: 5'- uUUUGUCGCAGCGCUugacCGguguuacuguucCAAGCGCGa -3' miRNA: 3'- cAAACAGCGUCGCGGu---GC------------GUUCGCGU- -5' |
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| 9399 | 5' | -55.6 | NC_002513.1 | + | 15344 | 1.08 | 0.000463 |
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Target: 5'- gGUUUGUCGCAGCGCCACGCAAGCGCAc -3' miRNA: 3'- -CAAACAGCGUCGCGGUGCGUUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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