Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 95 | 5' | -54.4 | AC_000006.1 | + | 19567 | 0.66 | 0.597411 |
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Target: 5'- --gGUGCAGUgGCGgcgcGGcGCGCGGCuCGCa -3' miRNA: 3'- gagUACGUUA-CGCa---CC-UGUGCCG-GUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 29405 | 0.67 | 0.540468 |
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Target: 5'- cCUCcuuUGUcuugGCGUGGACGCGcGCCu- -3' miRNA: 3'- -GAGu--ACGuua-CGCACCUGUGC-CGGug -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 17285 | 0.67 | 0.533746 |
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Target: 5'- --aGUGCAGcGCGagauaaacagccgucUGGACccGCGGCCGCc -3' miRNA: 3'- gagUACGUUaCGC---------------ACCUG--UGCCGGUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 17454 | 0.68 | 0.496265 |
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Target: 5'- ----gGCGG-GCGgGGACGCGGCgCACg -3' miRNA: 3'- gaguaCGUUaCGCaCCUGUGCCG-GUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 14594 | 0.68 | 0.485465 |
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Target: 5'- --gGUGCAGU-CGUGGACGCuGCuCACc -3' miRNA: 3'- gagUACGUUAcGCACCUGUGcCG-GUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 21302 | 0.7 | 0.38449 |
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Target: 5'- gUUGUGCGccGCGcUGGGgAgGGCCACg -3' miRNA: 3'- gAGUACGUuaCGC-ACCUgUgCCGGUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 12993 | 0.71 | 0.314377 |
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Target: 5'- -aCcgGCAGUGCccuGUGGGauCACGGCUGCa -3' miRNA: 3'- gaGuaCGUUACG---CACCU--GUGCCGGUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 574 | 0.71 | 0.306325 |
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Target: 5'- cCUCcUGCcuggaacuGUGCccuUGGACAUGGCCGCa -3' miRNA: 3'- -GAGuACGu-------UACGc--ACCUGUGCCGGUG- -5' |
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| 95 | 5' | -54.4 | AC_000006.1 | + | 26805 | 1.09 | 0.00062 |
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Target: 5'- gCUCAUGCAAUGCGUGGACACGGCCACc -3' miRNA: 3'- -GAGUACGUUACGCACCUGUGCCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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