Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9598 | 5' | -54.9 | NC_002577.1 | + | 2854 | 0.66 | 0.946534 |
Target: 5'- -cAGCga--CCGCGAUuuccucuGCGCCuaCCCu -3' miRNA: 3'- uaUCGaauaGGCGCUA-------UGCGGcgGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 130760 | 0.66 | 0.946534 |
Target: 5'- -cAGCga--CCGCGAUuuccucuGCGCCuaCCCu -3' miRNA: 3'- uaUCGaauaGGCGCUA-------UGCGGcgGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 104801 | 0.66 | 0.946971 |
Target: 5'- -cAGCUUGga-GCG-UGCGCCGUCUg -3' miRNA: 3'- uaUCGAAUaggCGCuAUGCGGCGGGg -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 135667 | 0.66 | 0.946971 |
Target: 5'- aAUAGgaccGUCUGCGAcguuUGCCGCUCCg -3' miRNA: 3'- -UAUCgaa-UAGGCGCUau--GCGGCGGGG- -5' |
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9598 | 5' | -54.9 | NC_002577.1 | + | 41752 | 0.66 | 0.946971 |
Target: 5'- --cGCUUcuguugggCCGCGAcACGUgGCCUCg -3' miRNA: 3'- uauCGAAua------GGCGCUaUGCGgCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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