Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 96 | 5' | -54.2 | AC_000006.1 | + | 19704 | 0.67 | 0.528635 |
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Target: 5'- cUCUUgCGUCuggcauaucGCCGc-CGCGUCGCCACc -3' miRNA: 3'- aAGAA-GCAG---------UGGCucGUGUAGCGGUG- -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 29530 | 0.69 | 0.432289 |
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Target: 5'- cUgUUUGUCACCGAGCGCGga--CACa -3' miRNA: 3'- aAgAAGCAGUGGCUCGUGUagcgGUG- -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 25831 | 0.69 | 0.402497 |
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Target: 5'- gUCgcgcCGUCACCGAaaccaUGCGUCGCCGCc -3' miRNA: 3'- aAGaa--GCAGUGGCUc----GUGUAGCGGUG- -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 25882 | 0.71 | 0.321351 |
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Target: 5'- -gCUUCGUCgACCGucucccGGUGCGUCGCCGu -3' miRNA: 3'- aaGAAGCAG-UGGC------UCGUGUAGCGGUg -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 28238 | 0.72 | 0.267145 |
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Target: 5'- gUUC-UCGUCaccGCCGAGUuCGUCGCCAa -3' miRNA: 3'- -AAGaAGCAG---UGGCUCGuGUAGCGGUg -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 25329 | 0.78 | 0.113595 |
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Target: 5'- ---aUCcUCGCCGAGCGCGUCGUCGCc -3' miRNA: 3'- aagaAGcAGUGGCUCGUGUAGCGGUG- -5' |
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| 96 | 5' | -54.2 | AC_000006.1 | + | 26185 | 1.06 | 0.000964 |
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Target: 5'- cUUCUUCGUCACCGAGCACAUCGCCACc -3' miRNA: 3'- -AAGAAGCAGUGGCUCGUGUAGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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