Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9600 | 3' | -54.2 | NC_002577.1 | + | 121938 | 0.66 | 0.967407 |
Target: 5'- cCUCCGgacGCCgagAgGGAUGCGgagcgcgaGAGCGGAg -3' miRNA: 3'- -GAGGC---CGGa--UgUUUAUGCg-------CUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 87319 | 0.66 | 0.967407 |
Target: 5'- cCUCuaCGGCCgagAUAAGUucGCGCGAGCc-- -3' miRNA: 3'- -GAG--GCCGGa--UGUUUA--UGCGCUCGccu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 11676 | 0.66 | 0.967407 |
Target: 5'- cCUCCGgacGCCgagAgGGAUGCGgagcgcgaGAGCGGAg -3' miRNA: 3'- -GAGGC---CGGa--UgUUUAUGCg-------CUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 10158 | 0.66 | 0.953175 |
Target: 5'- --aCGGCCUcucgaACGAGaGCGgGGGUGGGg -3' miRNA: 3'- gagGCCGGA-----UGUUUaUGCgCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 140798 | 0.66 | 0.953175 |
Target: 5'- -gUgGGCUUACAgggcgcgucaGAUACGC-AGCGGAc -3' miRNA: 3'- gaGgCCGGAUGU----------UUAUGCGcUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 123456 | 0.66 | 0.953175 |
Target: 5'- --aCGGCCUcucgaACGAGaGCGgGGGUGGGg -3' miRNA: 3'- gagGCCGGA-----UGUUUaUGCgCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 156539 | 0.66 | 0.953175 |
Target: 5'- -gUgGGCUUACAgggcgcgucaGAUACGC-AGCGGAc -3' miRNA: 3'- gaGgCCGGAUGU----------UUAUGCGcUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 145140 | 0.66 | 0.949051 |
Target: 5'- aUCCGGCC-ACAGAUGagauguucUGCGAaguuuGCGGc -3' miRNA: 3'- gAGGCCGGaUGUUUAU--------GCGCU-----CGCCu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 125566 | 0.67 | 0.940095 |
Target: 5'- gCUCCgGGCCggaGAGgcugGCGGGCGGGu -3' miRNA: 3'- -GAGG-CCGGaugUUUaug-CGCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 8047 | 0.67 | 0.940095 |
Target: 5'- gCUCCgGGCCggaGAGgcugGCGGGCGGGu -3' miRNA: 3'- -GAGG-CCGGaugUUUaug-CGCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 70628 | 0.67 | 0.940095 |
Target: 5'- -gCCGGagguaCUGCGcGAUGC-CGAGCGGGu -3' miRNA: 3'- gaGGCCg----GAUGU-UUAUGcGCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 9809 | 0.68 | 0.919306 |
Target: 5'- aUCCGGaaucCCgUGCAGGUACGCaccGCGGGc -3' miRNA: 3'- gAGGCC----GG-AUGUUUAUGCGcu-CGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 123804 | 0.68 | 0.919306 |
Target: 5'- aUCCGGaaucCCgUGCAGGUACGCaccGCGGGc -3' miRNA: 3'- gAGGCC----GG-AUGUUUAUGCGcu-CGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 21933 | 0.68 | 0.919306 |
Target: 5'- -aCCGGCCUccuucuucuAC-AGUACGCGGcguGCGGu -3' miRNA: 3'- gaGGCCGGA---------UGuUUAUGCGCU---CGCCu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 31423 | 0.69 | 0.887931 |
Target: 5'- -gCCGGUCUGCAcagauuAAUugGaaaCGGGCGGGc -3' miRNA: 3'- gaGGCCGGAUGU------UUAugC---GCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 57649 | 0.69 | 0.866348 |
Target: 5'- uCUCUGGCCaagaagaACGAGgccgGCGCGGGCGc- -3' miRNA: 3'- -GAGGCCGGa------UGUUUa---UGCGCUCGCcu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 10303 | 0.69 | 0.850887 |
Target: 5'- gUCUGGCCgGCucg-GCGCGAGcCGGc -3' miRNA: 3'- gAGGCCGGaUGuuuaUGCGCUC-GCCu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 123311 | 0.69 | 0.850887 |
Target: 5'- gUCUGGCCgGCucg-GCGCGAGcCGGc -3' miRNA: 3'- gAGGCCGGaUGuuuaUGCGCUC-GCCu -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 9184 | 0.7 | 0.834624 |
Target: 5'- uUUCGGUgUcugGCGAugGCGCGGGCGGGg -3' miRNA: 3'- gAGGCCGgA---UGUUuaUGCGCUCGCCU- -5' |
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9600 | 3' | -54.2 | NC_002577.1 | + | 153082 | 0.7 | 0.834624 |
Target: 5'- gCUCCGGguCUUACAc---CGCGAGCGGc -3' miRNA: 3'- -GAGGCC--GGAUGUuuauGCGCUCGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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