Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9602 | 5' | -55.7 | NC_002577.1 | + | 108170 | 0.67 | 0.899172 |
Target: 5'- cCUCG-CACGCgucggggGCuCCGUGGCgcaugcaguucuCCGUCGa -3' miRNA: 3'- -GAGCuGUGCGa------UG-GGUAUCG------------GGCAGC- -5' |
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9602 | 5' | -55.7 | NC_002577.1 | + | 151962 | 0.66 | 0.922806 |
Target: 5'- --aGACAUuaUGCCaCGUAGCCCGg-- -3' miRNA: 3'- gagCUGUGcgAUGG-GUAUCGGGCagc -5' |
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9602 | 5' | -55.7 | NC_002577.1 | + | 85480 | 0.66 | 0.922806 |
Target: 5'- aUCGACGCaGCUguagcgACCCugcUGGgCCGUCu -3' miRNA: 3'- gAGCUGUG-CGA------UGGGu--AUCgGGCAGc -5' |
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9602 | 5' | -55.7 | NC_002577.1 | + | 58609 | 0.66 | 0.928133 |
Target: 5'- aCUCG-CGCGUUGCCUGggacuuUAGCucuuCCGUCGu -3' miRNA: 3'- -GAGCuGUGCGAUGGGU------AUCG----GGCAGC- -5' |
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9602 | 5' | -55.7 | NC_002577.1 | + | 128505 | 0.66 | 0.94271 |
Target: 5'- uUCG-CGCGCgucGCCCu--GUCCGUCc -3' miRNA: 3'- gAGCuGUGCGa--UGGGuauCGGGCAGc -5' |
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9602 | 5' | -55.7 | NC_002577.1 | + | 5108 | 0.66 | 0.94271 |
Target: 5'- uUCG-CGCGCgucGCCCu--GUCCGUCc -3' miRNA: 3'- gAGCuGUGCGa--UGGGuauCGGGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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