Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9602 | 5' | -55.7 | NC_002577.1 | + | 129179 | 0.71 | 0.684243 |
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Target: 5'- aCUCGGCGCGCgUACCCGcGGCCa-UCc -3' miRNA: 3'- -GAGCUGUGCG-AUGGGUaUCGGgcAGc -5' |
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| 9602 | 5' | -55.7 | NC_002577.1 | + | 134991 | 0.71 | 0.684243 |
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Target: 5'- -gCGACAgCGCcuagaagACCCGUGGCCUGUaCGa -3' miRNA: 3'- gaGCUGU-GCGa------UGGGUAUCGGGCA-GC- -5' |
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| 9602 | 5' | -55.7 | NC_002577.1 | + | 4435 | 0.71 | 0.684243 |
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Target: 5'- aCUCGGCGCGCgUACCCGcGGCCa-UCc -3' miRNA: 3'- -GAGCUGUGCG-AUGGGUaUCGGgcAGc -5' |
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| 9602 | 5' | -55.7 | NC_002577.1 | + | 8891 | 0.72 | 0.664128 |
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Target: 5'- aUCG-CACGCauuCCCGgucGCCCGUCGc -3' miRNA: 3'- gAGCuGUGCGau-GGGUau-CGGGCAGC- -5' |
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| 9602 | 5' | -55.7 | NC_002577.1 | + | 124723 | 0.72 | 0.664128 |
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Target: 5'- aUCG-CACGCauuCCCGgucGCCCGUCGc -3' miRNA: 3'- gAGCuGUGCGau-GGGUau-CGGGCAGC- -5' |
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| 9602 | 5' | -55.7 | NC_002577.1 | + | 15737 | 1.09 | 0.003453 |
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Target: 5'- cCUCGACACGCUACCCAUAGCCCGUCGa -3' miRNA: 3'- -GAGCUGUGCGAUGGGUAUCGGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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