Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9603 | 3' | -60.2 | NC_002577.1 | + | 121773 | 0.71 | 0.510796 |
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Target: 5'- gGGGCGcgCGGaucagCGGUCGGGUCGcgauCGCGu -3' miRNA: 3'- -CUCGCa-GCUg----GCCAGCCCAGCu---GCGC- -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 19131 | 0.71 | 0.510796 |
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Target: 5'- -cGCGUCGACCcGaCcGGUCGACGCu -3' miRNA: 3'- cuCGCAGCUGGcCaGcCCAGCUGCGc -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 11840 | 0.71 | 0.510796 |
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Target: 5'- gGGGCGcgCGGaucagCGGUCGGGUCGcgauCGCGu -3' miRNA: 3'- -CUCGCa-GCUg----GCCAGCCCAGCu---GCGC- -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 130001 | 0.71 | 0.492451 |
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Target: 5'- cGGGCGcgaagCGGCUGGgaaUCGGGUCG-CGUGg -3' miRNA: 3'- -CUCGCa----GCUGGCC---AGCCCAGCuGCGC- -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 99132 | 0.72 | 0.473522 |
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Target: 5'- aGAaCGUCGACCGaGgCGGGUCGuugacacACGCGg -3' miRNA: 3'- -CUcGCAGCUGGC-CaGCCCAGC-------UGCGC- -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 3612 | 0.72 | 0.456716 |
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Target: 5'- cGGGCGcgaagCGGCUGGgaUCGGGUCG-CGUGg -3' miRNA: 3'- -CUCGCa----GCUGGCC--AGCCCAGCuGCGC- -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 12045 | 0.74 | 0.343885 |
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Target: 5'- cGGGuCGUCGcCCGGgucgucgccCGGGUCGGCGCc -3' miRNA: 3'- -CUC-GCAGCuGGCCa--------GCCCAGCUGCGc -5' |
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| 9603 | 3' | -60.2 | NC_002577.1 | + | 19321 | 1.08 | 0.002021 |
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Target: 5'- cGAGCGUCGACCGGUCGGGUCGACGCGu -3' miRNA: 3'- -CUCGCAGCUGGCCAGCCCAGCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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