miRNA: 3'- -GUCGUUg-UCGUGGU---UGUCcuGGUGGU- -5'
miRNA: 3'- -GUCGU---UGUCG-------UGGUuGUCCUGGUGgu -5'
miRNA: 3'- gUCGUUGucGUgGUUGUCCuGGUGGu -5'
miRNA: 3'- -GUCGuUGUCGUG-GuUGUCc--UGGUGGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 967 | 3' | -54.4 | NC_000902.1 | + | 33707 | 0.66 | 0.745799 |
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Target: 5'- aCAGCAGgaAGCGCCAaaaGCGGuuUCGCCGg -3' miRNA: 3'- -GUCGUUg-UCGUGGU---UGUCcuGGUGGU- -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 15362 | 0.66 | 0.755257 |
|
Target: 5'- aCGGUAauuGCAGUaaugaaaGCCAuCAGGGCCGCa- -3' miRNA: 3'- -GUCGU---UGUCG-------UGGUuGUCCUGGUGgu -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 7895 | 0.66 | 0.756302 |
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Target: 5'- gGGCAGC--CAgCAACAGG-CCACCc -3' miRNA: 3'- gUCGUUGucGUgGUUGUCCuGGUGGu -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 27141 | 0.66 | 0.766674 |
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Target: 5'- cCAGCcACAGCGCaCcAUAGccaACCGCCAg -3' miRNA: 3'- -GUCGuUGUCGUG-GuUGUCc--UGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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