miRNA: 3'- -GUCguUGU---CGUGGUU-GUCC------------UGGUGGu -5'
miRNA: 3'- -GUCGUUGuCGUGGUUguCCU-GGuGGU- -5'
miRNA: 3'- guCGUUGUCGUGGuuG------UCCUGGUGGU- -5'
miRNA: 3'- -GUCGUUG---UC-GUGGUuGUCC------UGGUGGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 967 | 3' | -54.4 | NC_000902.1 | + | 48706 | 0.67 | 0.713629 |
|
Target: 5'- uCAGguACAagcGCACCAAuCAGGuuuacacccuucGCCACCc -3' miRNA: 3'- -GUCguUGU---CGUGGUU-GUCC------------UGGUGGu -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 49389 | 0.67 | 0.702724 |
|
Target: 5'- cCGGCAACcGCACCGAauGGAgCCcCCAu -3' miRNA: 3'- -GUCGUUGuCGUGGUUguCCU-GGuGGU- -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 50511 | 0.78 | 0.168801 |
|
Target: 5'- -cGCGACAGCACCggUacgugaAGGGCUACCGg -3' miRNA: 3'- guCGUUGUCGUGGuuG------UCCUGGUGGU- -5' |
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| 967 | 3' | -54.4 | NC_000902.1 | + | 55722 | 0.68 | 0.647371 |
|
Target: 5'- cCGGCAGCacgGGaCGCCGuCAGGucacccGCCGCCAc -3' miRNA: 3'- -GUCGUUG---UC-GUGGUuGUCC------UGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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