Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9797 | 5' | -54.4 | NC_002593.1 | + | 71467 | 0.66 | 0.911407 |
Target: 5'- cGGGACCCA--ACGACG-UGCCgCUgGa -3' miRNA: 3'- -CCUUGGGUugUGCUGCgAUGGgGAgU- -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 37888 | 0.66 | 0.891798 |
Target: 5'- --cACCCGcCACGACGC-GCUgCUCGu -3' miRNA: 3'- ccuUGGGUuGUGCUGCGaUGGgGAGU- -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 81187 | 0.66 | 0.891798 |
Target: 5'- -uGAUCCGGCucaucguCGACGCUccAUCCCUUAa -3' miRNA: 3'- ccUUGGGUUGu------GCUGCGA--UGGGGAGU- -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 1466 | 0.68 | 0.829251 |
Target: 5'- ---uCCCGACAgggugUGGCGUUcCCCCUCAc -3' miRNA: 3'- ccuuGGGUUGU-----GCUGCGAuGGGGAGU- -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 1644 | 0.68 | 0.811557 |
Target: 5'- aGGAAUUCGcGCcCGACG-UACCCCUCu -3' miRNA: 3'- -CCUUGGGU-UGuGCUGCgAUGGGGAGu -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 90339 | 0.69 | 0.744671 |
Target: 5'- --uGCUCAACGCcacauACGcCUGCCCCUCGa -3' miRNA: 3'- ccuUGGGUUGUGc----UGC-GAUGGGGAGU- -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 50748 | 0.72 | 0.598873 |
Target: 5'- cGGcACCCAACACGGUGUUACCgCCg-- -3' miRNA: 3'- -CCuUGGGUUGUGCUGCGAUGG-GGagu -5' |
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9797 | 5' | -54.4 | NC_002593.1 | + | 67644 | 1.11 | 0.002079 |
Target: 5'- cGGAACCCAACACGACGCUACCCCUCAc -3' miRNA: 3'- -CCUUGGGUUGUGCUGCGAUGGGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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