Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9804 | 3' | -54 | NC_002593.1 | + | 99432 | 0.66 | 0.912395 |
Target: 5'- gACCacgucgguggUGGUGGCGgaCGAGACGACG-GUg -3' miRNA: 3'- gUGGg---------ACCACUGC--GCUUUGCUGCaCG- -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 31004 | 0.66 | 0.906151 |
Target: 5'- aCGCUUcGGUGcCGC-AGACGGCGcUGCa -3' miRNA: 3'- -GUGGGaCCACuGCGcUUUGCUGC-ACG- -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 30779 | 0.68 | 0.855678 |
Target: 5'- aCACCCUGccgagcGUGAgCGUGAAcguCGACGgGCc -3' miRNA: 3'- -GUGGGAC------CACU-GCGCUUu--GCUGCaCG- -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 100584 | 0.68 | 0.855678 |
Target: 5'- uUACCUUGGUaGACaGC--AACGGCGUGg -3' miRNA: 3'- -GUGGGACCA-CUG-CGcuUUGCUGCACg -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 13894 | 0.68 | 0.842589 |
Target: 5'- gACCUcGGUGguaaagagcuuguugGCGgGGAACGugGUGUc -3' miRNA: 3'- gUGGGaCCAC---------------UGCgCUUUGCugCACG- -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 8397 | 0.69 | 0.785374 |
Target: 5'- aCACCCcuaucGGUGACGCGAAACaGAgaGUa- -3' miRNA: 3'- -GUGGGa----CCACUGCGCUUUG-CUg-CAcg -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 12585 | 0.71 | 0.705761 |
Target: 5'- gCGCCUgGGUGACGaGGAGCGccuGCGUGg -3' miRNA: 3'- -GUGGGaCCACUGCgCUUUGC---UGCACg -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 37515 | 0.77 | 0.361321 |
Target: 5'- uCAUCCUGGUGACGUGguGCGGCa--- -3' miRNA: 3'- -GUGGGACCACUGCGCuuUGCUGcacg -5' |
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9804 | 3' | -54 | NC_002593.1 | + | 30839 | 1.11 | 0.002422 |
Target: 5'- cCACCCUGGUGACGCGAAACGACGUGCc -3' miRNA: 3'- -GUGGGACCACUGCGCUUUGCUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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