Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9812 | 3' | -52.7 | NC_002593.1 | + | 37941 | 0.66 | 0.95084 |
Target: 5'- gGUcgGCGCCGccuGGCGGCCaACGaCACc -3' miRNA: 3'- aCA--UGCGGUu--UUGCCGGcUGCaGUGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 37687 | 0.66 | 0.931508 |
Target: 5'- gUGUGCGUgauGAGCGGCgGuaaaggcguCGUCACUu -3' miRNA: 3'- -ACAUGCGgu-UUUGCCGgCu--------GCAGUGA- -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 99541 | 0.69 | 0.839568 |
Target: 5'- aGUACGUUgccacccugagcgAAAGCGGCCacaACGUCACc -3' miRNA: 3'- aCAUGCGG-------------UUUUGCCGGc--UGCAGUGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 70037 | 0.69 | 0.831782 |
Target: 5'- uUGUACGCCAAcuugagugguAugGGCCugaccGCGUCGg- -3' miRNA: 3'- -ACAUGCGGUU----------UugCCGGc----UGCAGUga -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 67726 | 0.69 | 0.822941 |
Target: 5'- --cACGCCGAcGACGGC-GACGUCGu- -3' miRNA: 3'- acaUGCGGUU-UUGCCGgCUGCAGUga -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 67312 | 0.69 | 0.822941 |
Target: 5'- aUGUGCGUaga---GGCgGGCGUCACg -3' miRNA: 3'- -ACAUGCGguuuugCCGgCUGCAGUGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 70180 | 1.09 | 0.004134 |
Target: 5'- cUGUACGCCAAAACGGCCGACGUCACUa -3' miRNA: 3'- -ACAUGCGGUUUUGCCGGCUGCAGUGA- -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 31491 | 0.72 | 0.673056 |
Target: 5'- --gACGCCAAcACGGCCGAUuUCAg- -3' miRNA: 3'- acaUGCGGUUuUGCCGGCUGcAGUga -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 79719 | 0.72 | 0.683693 |
Target: 5'- uUGUACGCCcugcGAACcacGCCGACGUC-CUu -3' miRNA: 3'- -ACAUGCGGu---UUUGc--CGGCUGCAGuGA- -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 69261 | 0.7 | 0.81391 |
Target: 5'- cGUACGCCGAcagauuuuACGGaaGACGUCugaGCg -3' miRNA: 3'- aCAUGCGGUUu-------UGCCggCUGCAG---UGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 50835 | 0.69 | 0.840423 |
Target: 5'- cGUAUGCgGAaaaaaugauGACGuacuuugucagcGCCGGCGUCACUu -3' miRNA: 3'- aCAUGCGgUU---------UUGC------------CGGCUGCAGUGA- -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 30459 | 0.67 | 0.920274 |
Target: 5'- cUGUACuGCUGGAAC-GCCGGCGaUUACg -3' miRNA: 3'- -ACAUG-CGGUUUUGcCGGCUGC-AGUGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 36284 | 0.67 | 0.926022 |
Target: 5'- cGU-CGUCuucGGGCGGCggCGGCGUCACc -3' miRNA: 3'- aCAuGCGGu--UUUGCCG--GCUGCAGUGa -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 37817 | 0.67 | 0.926022 |
Target: 5'- cGUugGCCGccAGGCGGCgcCGACcagcUCGCUu -3' miRNA: 3'- aCAugCGGU--UUUGCCG--GCUGc---AGUGA- -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 29904 | 0.66 | 0.93673 |
Target: 5'- cGUccACGCCAAggugugcaugguGACGGCaGACGUCc-- -3' miRNA: 3'- aCA--UGCGGUU------------UUGCCGgCUGCAGuga -5' |
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9812 | 3' | -52.7 | NC_002593.1 | + | 29435 | 0.66 | 0.946394 |
Target: 5'- cGUGCGCCAcGACGcgacccucGCCGucgaacggguaaGCGUCAa- -3' miRNA: 3'- aCAUGCGGUuUUGC--------CGGC------------UGCAGUga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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