Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9987 | 3' | -52.1 | NC_002654.1 | + | 75102 | 0.66 | 0.980615 |
Target: 5'- cAGUGgACguUCGGCGACGCaCACUuuACu -3' miRNA: 3'- -UCAC-UGguAGUUGCUGCG-GUGGu-UGu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 35962 | 0.66 | 0.980615 |
Target: 5'- cGUGAUCGgucCGACGgcuACGCCACUGAa- -3' miRNA: 3'- uCACUGGUa--GUUGC---UGCGGUGGUUgu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 95221 | 0.66 | 0.978311 |
Target: 5'- --aGaACCAUgaggaAACGuuGCCACCAACAg -3' miRNA: 3'- ucaC-UGGUAg----UUGCugCGGUGGUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 14866 | 0.66 | 0.97581 |
Target: 5'- gAGUca-CAUUAugGAUGCCGCgAACAu -3' miRNA: 3'- -UCAcugGUAGUugCUGCGGUGgUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 119787 | 0.66 | 0.97581 |
Target: 5'- uGUGACCGUCAACaacaagaguCGCUACacaGAUAa -3' miRNA: 3'- uCACUGGUAGUUGcu-------GCGGUGg--UUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 21794 | 0.66 | 0.973105 |
Target: 5'- cGUGGCCAgCGACGGCacuacaccuuugGCCAgaGACAg -3' miRNA: 3'- uCACUGGUaGUUGCUG------------CGGUggUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 10309 | 0.66 | 0.96705 |
Target: 5'- --cGACUguaGUCGACGACGCCAUa---- -3' miRNA: 3'- ucaCUGG---UAGUUGCUGCGGUGguugu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 17051 | 0.66 | 0.96705 |
Target: 5'- cGUGGuCCAagAAUGGCGUaGCCAACGc -3' miRNA: 3'- uCACU-GGUagUUGCUGCGgUGGUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 36237 | 0.67 | 0.963686 |
Target: 5'- cGUGGCCGUgGACGcuuUGCuCGCgCAACGa -3' miRNA: 3'- uCACUGGUAgUUGCu--GCG-GUG-GUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 45693 | 0.67 | 0.956254 |
Target: 5'- cAG-GACaauUCAACGACGUCAUCGAa- -3' miRNA: 3'- -UCaCUGgu-AGUUGCUGCGGUGGUUgu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 85789 | 0.67 | 0.952176 |
Target: 5'- uGUGAUaaguUCAACGAuCGUCACCAuguCAg -3' miRNA: 3'- uCACUGgu--AGUUGCU-GCGGUGGUu--GU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 17001 | 0.68 | 0.943273 |
Target: 5'- --cGACCGUCGcCGGuguUGCCACCGAa- -3' miRNA: 3'- ucaCUGGUAGUuGCU---GCGGUGGUUgu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 113278 | 0.68 | 0.943273 |
Target: 5'- cAGUuuuaaauCCAUUAAgGACGUCAUCAACAg -3' miRNA: 3'- -UCAcu-----GGUAGUUgCUGCGGUGGUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 32589 | 0.68 | 0.922406 |
Target: 5'- gGGUG-UCAUgAACGACGCCAaaGACc -3' miRNA: 3'- -UCACuGGUAgUUGCUGCGGUggUUGu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 48670 | 0.69 | 0.910429 |
Target: 5'- uGGUGaAUCAUCgGGCGugGCCGCaAACGc -3' miRNA: 3'- -UCAC-UGGUAG-UUGCugCGGUGgUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 59150 | 0.69 | 0.906636 |
Target: 5'- --cGACaaCAUCGGCGGgucucacgccgucuuCGCCGCCGACAc -3' miRNA: 3'- ucaCUG--GUAGUUGCU---------------GCGGUGGUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 60943 | 0.69 | 0.904057 |
Target: 5'- cGGUGACCucggGUCGcaaACGuaACGCCGCCGGa- -3' miRNA: 3'- -UCACUGG----UAGU---UGC--UGCGGUGGUUgu -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 46484 | 0.69 | 0.890559 |
Target: 5'- uGUGGCgAUagCAGuCGACGCCAUgGACAu -3' miRNA: 3'- uCACUGgUA--GUU-GCUGCGGUGgUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 74946 | 0.7 | 0.868488 |
Target: 5'- aAGUGugCGUCGcCGaACGuCCACUGACGu -3' miRNA: 3'- -UCACugGUAGUuGC-UGC-GGUGGUUGU- -5' |
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9987 | 3' | -52.1 | NC_002654.1 | + | 115216 | 0.72 | 0.79072 |
Target: 5'- cGUGA-CAUUAACGACGCCGUCAAUc -3' miRNA: 3'- uCACUgGUAGUUGCUGCGGUGGUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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