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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 91341 | 0.69 | 0.972521 |
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Target: 5'- gGGCaCCGGGGCUGGcgUUAGGg-- -3' miRNA: 3'- -UUGaGGCCCCGACU--AGUCCaau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 91853 | 0.69 | 0.975416 |
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Target: 5'- gGGCUUCaGGGGCaUGGUCGGGc-- -3' miRNA: 3'- -UUGAGG-CCCCG-ACUAGUCCaau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 133999 | 0.7 | 0.940153 |
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Target: 5'- uGC-CCGGGGCggcgGGUCGGGa-- -3' miRNA: 3'- uUGaGGCCCCGa---CUAGUCCaau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | - | 2864 | 0.7 | 0.949869 |
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Target: 5'- uGCUCCGGGGCcgguggcGGUCuGGUa- -3' miRNA: 3'- uUGAGGCCCCGa------CUAGuCCAau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 55698 | 0.7 | 0.949869 |
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Target: 5'- uGGCUUCGGGGUUG-UCGuGGUUGg -3' miRNA: 3'- -UUGAGGCCCCGACuAGU-CCAAU- -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 5315 | 0.71 | 0.934869 |
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Target: 5'- -cCUCCGGGGCUGcguccugccuuGUUGGGUg- -3' miRNA: 3'- uuGAGGCCCCGAC-----------UAGUCCAau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | - | 116400 | 0.72 | 0.906868 |
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Target: 5'- -uCUCCGGGGUcagcgaggcccucuuUGAUCAGGa-- -3' miRNA: 3'- uuGAGGCCCCG---------------ACUAGUCCaau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | + | 170651 | 0.74 | 0.83169 |
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Target: 5'- gGGCUCCGGGGCUG--CGGGc-- -3' miRNA: 3'- -UUGAGGCCCCGACuaGUCCaau -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | - | 102150 | 0.75 | 0.762907 |
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Target: 5'- aGACUCCGGGGUUGcggAGGUUGg -3' miRNA: 3'- -UUGAGGCCCCGACuagUCCAAU- -5' |
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| MIMAT0000995 | ebv-miR-BHRF1-1 | -48.6 | NC_007605.1 | - | 41626 | 1.02 | 0.026909 |
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Target: 5'- cAACUCCGGGGCUGAUCAGGUUAa -3' miRNA: 3'- -UUGAGGCCCCGACUAGUCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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