miRNA display CGI


Results 21 - 26 of 26 are showing below:
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Acc. No. Name Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 221807 0.69 0.962831
Target:  5'- cGGcCCGGGCCGCccGGCGGg- -3'
miRNA:   3'- -CCaGGCUCGGUGacUCGCCaa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 142386 0.69 0.959075
Target:  5'- -uUCCGcAGCCGCUcAGCGGUa -3'
miRNA:   3'- ccAGGC-UCGGUGAcUCGCCAa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 - 95472 0.7 0.945803
Target:  5'- gGGUCCGAggcGCCgaccccgGCUGGGCGGc- -3'
miRNA:   3'- -CCAGGCU---CGG-------UGACUCGCCaa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 87606 0.71 0.919591
Target:  5'- cGUCCGucGCCGCUGuugcGGCGGUa -3'
miRNA:   3'- cCAGGCu-CGGUGAC----UCGCCAa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 - 145172 0.74 0.791512
Target:  5'- cGGUUCGGGCgGCuUGAGCGGg- -3'
miRNA:   3'- -CCAGGCUCGgUG-ACUCGCCaa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 221296 1 0.03163
Target:  5'- cGGUCCGAGCCACUGAGCGGUUc -3'
miRNA:   3'- -CCAGGCUCGGUGACUCGCCAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.