Results 21 - 26 of 26 are showing below:
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| Acc. No. | Name | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | - | 199128 | 0.66 | 0.995714 |
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Target: 5'- cGGUCCGucgagaccggcGGaCCGCgGAGCGGa- -3' miRNA: 3'- -CCAGGC-----------UC-GGUGaCUCGCCaa -5' |
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| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | - | 214139 | 0.68 | 0.982619 |
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Target: 5'- cGGUggCCGAGCUAUgcGAGCGGg- -3' miRNA: 3'- -CCA--GGCUCGGUGa-CUCGCCaa -5' |
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| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | + | 221296 | 1 | 0.03163 |
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Target: 5'- cGGUCCGAGCCACUGAGCGGUUc -3' miRNA: 3'- -CCAGGCUCGGUGACUCGCCAA- -5' |
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| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | + | 221807 | 0.69 | 0.962831 |
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Target: 5'- cGGcCCGGGCCGCccGGCGGg- -3' miRNA: 3'- -CCaGGCUCGGUGacUCGCCaa -5' |
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| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | + | 231175 | 0.66 | 0.995714 |
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Target: 5'- gGGUCCGAGCUGCgGuaugggucacGGCGGc- -3' miRNA: 3'- -CCAGGCUCGGUGaC----------UCGCCaa -5' |
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| MIMAT0001581 | hcmv-miR-US25-1 | -50 | NC_006273.1 | - | 233095 | 0.66 | 0.994132 |
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Target: 5'- cGUCCGGGCCGCggcGAGgcugaGGUg -3' miRNA: 3'- cCAGGCUCGGUGa--CUCg----CCAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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