miRNA display CGI


Results 21 - 26 of 26 are showing below:
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Acc. No. Name Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 - 70227 0.66 0.996363
Target:  5'- ---gCGGGCCAUUGGGUGGg- -3'
miRNA:   3'- ccagGCUCGGUGACUCGCCaa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 68404 0.66 0.99318
Target:  5'- uGGUCaCGcGCCACaaaaUGAGCGGc- -3'
miRNA:   3'- -CCAG-GCuCGGUG----ACUCGCCaa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 - 64249 0.68 0.984616
Target:  5'- -aUCUGGGCCACcGuGCGGUg -3'
miRNA:   3'- ccAGGCUCGGUGaCuCGCCAa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 61259 0.69 0.962831
Target:  5'- aGGUCC-AGCCACUGcAGCGcGg- -3'
miRNA:   3'- -CCAGGcUCGGUGAC-UCGC-Caa -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 - 35510 0.66 0.994973
Target:  5'- uGG-CCGAGaCGCUGcGCGGUUu -3'
miRNA:   3'- -CCaGGCUCgGUGACuCGCCAA- -5'
MIMAT0001581 hcmv-miR-US25-1 -50 NC_006273.1 + 15976 0.67 0.990909
Target:  5'- uGUCCGGGCCACaGGGaGGg- -3'
miRNA:   3'- cCAGGCUCGGUGaCUCgCCaa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.