Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1022 | 3' | -54.4 | NC_000924.1 | + | 11930 | 0.72 | 0.426875 |
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Target: 5'- uCGGCgAACAGCGCCccguguGGCAGGGCaauCCAg -3' miRNA: 3'- -GUCG-UUGUCGUGG------UUGUCCUGgu-GGU- -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 24096 | 0.72 | 0.417268 |
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Target: 5'- aGGgAACAcCGCCAGCAGaGCCACCGa -3' miRNA: 3'- gUCgUUGUcGUGGUUGUCcUGGUGGU- -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 28315 | 0.72 | 0.389258 |
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Target: 5'- aCAGCAGUAGCACCAA-AGGAaaCCAUCAc -3' miRNA: 3'- -GUCGUUGUCGUGGUUgUCCU--GGUGGU- -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 48558 | 0.74 | 0.312942 |
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Target: 5'- gAGCGAUAGCcuuAUCAACGGGuAUCGCCAg -3' miRNA: 3'- gUCGUUGUCG---UGGUUGUCC-UGGUGGU- -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 38375 | 0.74 | 0.305198 |
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Target: 5'- aCAGUgcaaaGACGGCaACCAGCAGGGCgGCUg -3' miRNA: 3'- -GUCG-----UUGUCG-UGGUUGUCCUGgUGGu -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 51469 | 0.78 | 0.170469 |
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Target: 5'- -cGCGACAGCACCggUacgugaAGGGCUACCGg -3' miRNA: 3'- guCGUUGUCGUGGuuG------UCCUGGUGGU- -5' |
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| 1022 | 3' | -54.4 | NC_000924.1 | + | 47695 | 1.09 | 0.001218 |
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Target: 5'- cCAGCAACAGCACCAACAGGACCACCAa -3' miRNA: 3'- -GUCGUUGUCGUGGUUGUCCUGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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