Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10283 | 3' | -58.9 | NC_002687.1 | + | 101159 | 0.66 | 0.935939 |
Target: 5'- aGUGGUgUGACCGAaauguCGCCGAU--UGCCAg -3' miRNA: 3'- -CACCG-GCUGGCU-----GUGGCUAgcACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 199798 | 0.66 | 0.92493 |
Target: 5'- gGUGGUggCGAUUGGCACCGAgCGUaauuuauuacgacgGCCAc -3' miRNA: 3'- -CACCG--GCUGGCUGUGGCUaGCA--------------CGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 268315 | 0.66 | 0.919803 |
Target: 5'- -cGGCCGACCucaaaggcaaccugGAUACCGA--GUGCUu -3' miRNA: 3'- caCCGGCUGG--------------CUGUGGCUagCACGGu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 224389 | 0.67 | 0.916092 |
Target: 5'- --uGCCGauGCCGAUGCCGAUgcCGaUGCCGa -3' miRNA: 3'- cacCGGC--UGGCUGUGGCUA--GC-ACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 29929 | 0.67 | 0.910616 |
Target: 5'- -aGGCaGGCauCGGCuuuCCGAUCGUGCUg -3' miRNA: 3'- caCCGgCUG--GCUGu--GGCUAGCACGGu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 211025 | 0.67 | 0.904936 |
Target: 5'- cGUGGCCGGCCu-CugCGAccaGUGCa- -3' miRNA: 3'- -CACCGGCUGGcuGugGCUag-CACGgu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 209796 | 0.67 | 0.904357 |
Target: 5'- -gGGCCGAUCGGCGucuUUGAUCGagauuuggagagcUGCCGc -3' miRNA: 3'- caCCGGCUGGCUGU---GGCUAGC-------------ACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 98160 | 0.67 | 0.899054 |
Target: 5'- -cGGU--GCCGAUgguGCCGGUgGUGCCAg -3' miRNA: 3'- caCCGgcUGGCUG---UGGCUAgCACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 151560 | 0.67 | 0.899054 |
Target: 5'- -aGGCUGcuucucuauUCGACACUcGUCGUGCCGa -3' miRNA: 3'- caCCGGCu--------GGCUGUGGcUAGCACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 97800 | 0.67 | 0.892972 |
Target: 5'- cUGGCCcACCGGCACC-AUCG-GCa- -3' miRNA: 3'- cACCGGcUGGCUGUGGcUAGCaCGgu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 84518 | 0.67 | 0.889227 |
Target: 5'- gGUGGCuucaCGcucgguacagacgucACCGGCAUCG-UCGUGCCGu -3' miRNA: 3'- -CACCG----GC---------------UGGCUGUGGCuAGCACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 215666 | 0.68 | 0.880219 |
Target: 5'- cGUGGCCGucacugucGCCGuCGCCGAgccuaucgcCGUcGCCGa -3' miRNA: 3'- -CACCGGC--------UGGCuGUGGCUa--------GCA-CGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 172800 | 0.68 | 0.880219 |
Target: 5'- --aGCacaacaGGCCGGCACCGAUUGUGaCAa -3' miRNA: 3'- cacCGg-----CUGGCUGUGGCUAGCACgGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 238689 | 0.68 | 0.876243 |
Target: 5'- -gGGCC-ACCGACGCUGcgCGUgaaacucuuguggauGCCAu -3' miRNA: 3'- caCCGGcUGGCUGUGGCuaGCA---------------CGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 297990 | 0.68 | 0.873555 |
Target: 5'- -cGGCCGccaccgcuuCCGACuggcgaggcgGCCGcuuUCGUGCCAa -3' miRNA: 3'- caCCGGCu--------GGCUG----------UGGCu--AGCACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 145747 | 0.68 | 0.873555 |
Target: 5'- cUGGCCG-CCGACAagaucCCGAUCaacacgGCCu -3' miRNA: 3'- cACCGGCuGGCUGU-----GGCUAGca----CGGu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 87761 | 0.68 | 0.873555 |
Target: 5'- -cGGCCuGAUCGGCGCCGAacagcUCgGUGgCAg -3' miRNA: 3'- caCCGG-CUGGCUGUGGCU-----AG-CACgGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 298057 | 0.68 | 0.866705 |
Target: 5'- -aGGCCGcgGCCGcCGCCGcgcgCGUGCUu -3' miRNA: 3'- caCCGGC--UGGCuGUGGCua--GCACGGu -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 269893 | 0.68 | 0.866705 |
Target: 5'- -gGGUCGAacagccacgccUUGGCACUGGUgGUGCCAa -3' miRNA: 3'- caCCGGCU-----------GGCUGUGGCUAgCACGGU- -5' |
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10283 | 3' | -58.9 | NC_002687.1 | + | 328257 | 0.69 | 0.829821 |
Target: 5'- uUGGUgGACaaCGuCGgCGAUCGUGCCAc -3' miRNA: 3'- cACCGgCUG--GCuGUgGCUAGCACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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