Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10283 | 3' | -58.9 | NC_002687.1 | + | 328257 | 0.69 | 0.829821 |
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Target: 5'- uUGGUgGACaaCGuCGgCGAUCGUGCCAc -3' miRNA: 3'- cACCGgCUG--GCuGUgGCUAGCACGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 154276 | 0.69 | 0.829821 |
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Target: 5'- cGUGGCCGACacgcggugUGACAUUGAUgGUGUgGa -3' miRNA: 3'- -CACCGGCUG--------GCUGUGGCUAgCACGgU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 72924 | 0.69 | 0.821956 |
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Target: 5'- cGUGuGCCGacgccgccgccGCCGACacaccGCCGAgcaGUGCCAg -3' miRNA: 3'- -CAC-CGGC-----------UGGCUG-----UGGCUag-CACGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 299259 | 0.7 | 0.77187 |
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Target: 5'- -cGGCCGACUGGCGCCuGAauuccUUG-GCCGc -3' miRNA: 3'- caCCGGCUGGCUGUGG-CU-----AGCaCGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 312766 | 0.7 | 0.745279 |
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Target: 5'- aUGGCCG-CCGuCGCgGAUaccUGUGCCAc -3' miRNA: 3'- cACCGGCuGGCuGUGgCUA---GCACGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 291265 | 0.7 | 0.745279 |
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Target: 5'- cGUGGCCGACUGcACuGCCGAagUGaGCCAg -3' miRNA: 3'- -CACCGGCUGGC-UG-UGGCUa-GCaCGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 28108 | 0.71 | 0.717919 |
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Target: 5'- -cGGUCGAa-GACACgCGAUCGUGCUu -3' miRNA: 3'- caCCGGCUggCUGUG-GCUAGCACGGu -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 316044 | 0.71 | 0.708662 |
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Target: 5'- cUGGCgGugguGCCGAUgguGCCGAUgGUGCCGg -3' miRNA: 3'- cACCGgC----UGGCUG---UGGCUAgCACGGU- -5' |
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| 10283 | 3' | -58.9 | NC_002687.1 | + | 2732 | 1.08 | 0.004631 |
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Target: 5'- cGUGGCCGACCGACACCGAUCGUGCCAc -3' miRNA: 3'- -CACCGGCUGGCUGUGGCUAGCACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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