Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10286 | 3' | -53.5 | NC_002687.1 | + | 200802 | 0.66 | 0.996501 |
Target: 5'- uGCGUCugcacCGAgGcGCACGaggGCGCGCa -3' miRNA: 3'- gUGCAGua---GCUgCuCGUGUa--CGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 67876 | 0.66 | 0.996501 |
Target: 5'- gGgGUCgaacuugcuGUUGACGuGCACAacGCGCGCa -3' miRNA: 3'- gUgCAG---------UAGCUGCuCGUGUa-CGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 143030 | 0.66 | 0.996501 |
Target: 5'- gCACGUCGaUGAagcccuCGGGgGCAUGCGCc- -3' miRNA: 3'- -GUGCAGUaGCU------GCUCgUGUACGCGug -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 41911 | 0.66 | 0.995933 |
Target: 5'- gACGUCAUUGGCccuuccaugGAugGCGUGCGCAa -3' miRNA: 3'- gUGCAGUAGCUG---------CUcgUGUACGCGUg -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 330950 | 0.66 | 0.995291 |
Target: 5'- cCACGUaCGUgaaucgccaCGACGGcuGCcgGCAUGUGCACg -3' miRNA: 3'- -GUGCA-GUA---------GCUGCU--CG--UGUACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 85351 | 0.66 | 0.995291 |
Target: 5'- -cUGUUggCGAUGAGCGCGcacgGCGCGa -3' miRNA: 3'- guGCAGuaGCUGCUCGUGUa---CGCGUg -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 283544 | 0.66 | 0.995223 |
Target: 5'- uCACGagggggcUCG-CGACacuauGCACAUGUGCACg -3' miRNA: 3'- -GUGC-------AGUaGCUGcu---CGUGUACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 145484 | 0.66 | 0.99457 |
Target: 5'- gCGCGcC-UCGGCGAGCAgAUcggcaaguucGCGUACu -3' miRNA: 3'- -GUGCaGuAGCUGCUCGUgUA----------CGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 110415 | 0.66 | 0.99457 |
Target: 5'- -cCGUCGUCGAC-AGCACAcacgacaucUaCGCACu -3' miRNA: 3'- guGCAGUAGCUGcUCGUGU---------AcGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 233889 | 0.66 | 0.993762 |
Target: 5'- aGCaGUUGgaaaaGGCG-GCACAUGCGUACg -3' miRNA: 3'- gUG-CAGUag---CUGCuCGUGUACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 129786 | 0.66 | 0.993762 |
Target: 5'- uGCGUCGUUccCGGGCGUgAUGUGCACa -3' miRNA: 3'- gUGCAGUAGcuGCUCGUG-UACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 76333 | 0.66 | 0.993762 |
Target: 5'- gCACGUCgguguccagcauGUCGACGucaaAGUACAUGCagaaCACg -3' miRNA: 3'- -GUGCAG------------UAGCUGC----UCGUGUACGc---GUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 200536 | 0.66 | 0.993762 |
Target: 5'- gCAUGUCAaCGACGcgguGCACAgucgccucCGCACa -3' miRNA: 3'- -GUGCAGUaGCUGCu---CGUGUac------GCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 65870 | 0.67 | 0.990744 |
Target: 5'- cCACGUCAagGACGAGCugGUagGUAa -3' miRNA: 3'- -GUGCAGUagCUGCUCGugUAcgCGUg -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 80789 | 0.67 | 0.989516 |
Target: 5'- aACGUCGguUCG-CGGGCGCAgGUaauGCACg -3' miRNA: 3'- gUGCAGU--AGCuGCUCGUGUaCG---CGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 23744 | 0.67 | 0.989516 |
Target: 5'- -cUGU-GUCGAgGAGCugAuggUGCGCACa -3' miRNA: 3'- guGCAgUAGCUgCUCGugU---ACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 17370 | 0.67 | 0.989516 |
Target: 5'- gGCaGUCcggCGGCGuuuuGCGCA-GCGCACa -3' miRNA: 3'- gUG-CAGua-GCUGCu---CGUGUaCGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 123426 | 0.67 | 0.989124 |
Target: 5'- gACGUCgacGUCcAUGAGCAUgugaauggccucggAUGCGCACc -3' miRNA: 3'- gUGCAG---UAGcUGCUCGUG--------------UACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 198660 | 0.67 | 0.988164 |
Target: 5'- gGCGuUCAcgagccUCGAaguggGAGCGCucAUGCGCACa -3' miRNA: 3'- gUGC-AGU------AGCUg----CUCGUG--UACGCGUG- -5' |
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10286 | 3' | -53.5 | NC_002687.1 | + | 323374 | 0.68 | 0.986682 |
Target: 5'- -gUGUCAUCGcaAUGAGCACggGuCGCAg -3' miRNA: 3'- guGCAGUAGC--UGCUCGUGuaC-GCGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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