Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10291 | 5' | -55.6 | NC_002687.1 | + | 28450 | 0.66 | 0.982996 |
Target: 5'- -uUCGACCGcuuUGAGCaUGCUGaugcgCUGCGa -3' miRNA: 3'- cuAGCUGGC---ACUCGcGUGACa----GACGC- -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 5679 | 0.66 | 0.980884 |
Target: 5'- -cUCGACCaGUcGAGCgagGCGCUGacauauuUCUGCGu -3' miRNA: 3'- cuAGCUGG-CA-CUCG---CGUGAC-------AGACGC- -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 199145 | 0.67 | 0.962962 |
Target: 5'- -cUCGugUGUGGGCGUACUGgagucCUGa- -3' miRNA: 3'- cuAGCugGCACUCGCGUGACa----GACgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 17139 | 0.68 | 0.948402 |
Target: 5'- cGAUCGACCaUGAGCGCACUcUUUc-- -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAcAGAcgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 327303 | 0.68 | 0.948402 |
Target: 5'- -uUCGACCGaguugaaaaUGAGCuGCG-UGUCUGUGg -3' miRNA: 3'- cuAGCUGGC---------ACUCG-CGUgACAGACGC- -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 14827 | 0.69 | 0.930475 |
Target: 5'- cGAUCGACCaUGAGCGCACUa------ -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAcagacgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 16543 | 0.69 | 0.930475 |
Target: 5'- cGAUCGACCaUGAGCGCACUa------ -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAcagacgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 16878 | 0.69 | 0.930475 |
Target: 5'- cGAUCGACCaUGAGCGCACUa------ -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAcagacgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 17209 | 0.69 | 0.930475 |
Target: 5'- cGAUCGACCaUGAGCGCACUa------ -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAcagacgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 127934 | 0.69 | 0.930475 |
Target: 5'- aGUCGAuagauuugccuaCgGUGcauccGCGCACUGUCUGCa -3' miRNA: 3'- cUAGCU------------GgCACu----CGCGUGACAGACGc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 265964 | 0.69 | 0.925454 |
Target: 5'- aGAUCGugUagauGUgcGAGUGCugUGUUUGCGa -3' miRNA: 3'- -CUAGCugG----CA--CUCGCGugACAGACGC- -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 213422 | 0.72 | 0.822852 |
Target: 5'- gGAUCGACCGUGAGCacggcgGCAUUGgUaagagagagcagagCUGCGa -3' miRNA: 3'- -CUAGCUGGCACUCG------CGUGAC-A--------------GACGC- -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 16282 | 0.72 | 0.800359 |
Target: 5'- cGAUCGACCaUGAGgGCACUGUUUc-- -3' miRNA: 3'- -CUAGCUGGcACUCgCGUGACAGAcgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 15424 | 0.72 | 0.800359 |
Target: 5'- cGAUCGACCaUGAGgGCACUGUUUc-- -3' miRNA: 3'- -CUAGCUGGcACUCgCGUGACAGAcgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 14566 | 0.72 | 0.800359 |
Target: 5'- cGAUCGACCaUGAGgGCACUGUUUc-- -3' miRNA: 3'- -CUAGCUGGcACUCgCGUGACAGAcgc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 15354 | 0.78 | 0.498385 |
Target: 5'- cGAUCGACCaUGAGCGCACUauuucuucUCUGCa -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAc-------AGACGc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 15686 | 0.78 | 0.498385 |
Target: 5'- cGAUCGACCaUGAGCGCACUauuucuucUCUGCa -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAc-------AGACGc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 16213 | 0.78 | 0.498385 |
Target: 5'- cGAUCGACCaUGAGCGCACUauuucuucUCUGCa -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAc-------AGACGc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 14496 | 0.78 | 0.498385 |
Target: 5'- cGAUCGACCaUGAGCGCACUauuucuucUCUGCa -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAc-------AGACGc -5' |
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10291 | 5' | -55.6 | NC_002687.1 | + | 15616 | 0.82 | 0.305942 |
Target: 5'- cGAUCGACCaUGAGCGCACUcucCUGCGg -3' miRNA: 3'- -CUAGCUGGcACUCGCGUGAca-GACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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