Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10300 | 3' | -58.1 | NC_002687.1 | + | 211991 | 0.66 | 0.95243 |
Target: 5'- ---aGAUugGGCUUGCGGUguacgaGgGCg -3' miRNA: 3'- guagCUGugCCGAACGCCGag----CgCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 224111 | 0.66 | 0.95243 |
Target: 5'- ---gGACGCGGUggcgGUGGCgcugGCGCUg -3' miRNA: 3'- guagCUGUGCCGaa--CGCCGag--CGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 23967 | 0.66 | 0.948936 |
Target: 5'- uGUCGugGCGGCgugguggUGUGGCagcagugacgcagaGCGCa -3' miRNA: 3'- gUAGCugUGCCGa------ACGCCGag------------CGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 180784 | 0.66 | 0.948537 |
Target: 5'- gCAUCGGCGaagaagaucuGCUUGCGuacaacgagguuGCUCGUGCUg -3' miRNA: 3'- -GUAGCUGUgc--------CGAACGC------------CGAGCGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 200098 | 0.66 | 0.944017 |
Target: 5'- gGUCGACAugcucguuggugcCGGCgacGaCGGC-CGCGCg -3' miRNA: 3'- gUAGCUGU-------------GCCGaa-C-GCCGaGCGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 257965 | 0.66 | 0.930887 |
Target: 5'- gGUCGACAUGGUcaaCGGCaagcaaguacaUCGUGCUg -3' miRNA: 3'- gUAGCUGUGCCGaacGCCG-----------AGCGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 12260 | 0.67 | 0.925948 |
Target: 5'- gGUCGcgaaACugGGCgugacGCGGCU-GCGCa -3' miRNA: 3'- gUAGC----UGugCCGaa---CGCCGAgCGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 208454 | 0.67 | 0.920798 |
Target: 5'- gGUCGACGUGGUgccuucGCGGgucguuCUCGCGCUu -3' miRNA: 3'- gUAGCUGUGCCGaa----CGCC------GAGCGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 135619 | 0.67 | 0.920798 |
Target: 5'- aCcgCGACGCGGUgcaGCGGUUUG-GCa -3' miRNA: 3'- -GuaGCUGUGCCGaa-CGCCGAGCgCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 295131 | 0.67 | 0.915436 |
Target: 5'- cCAUCGGCGgauuGCUUGCGGaaCUCGCGa- -3' miRNA: 3'- -GUAGCUGUgc--CGAACGCC--GAGCGCga -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 325816 | 0.67 | 0.909864 |
Target: 5'- cCGUCGACG-GGuCUcGUGGCgggUGCGCUa -3' miRNA: 3'- -GUAGCUGUgCC-GAaCGCCGa--GCGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 169818 | 0.68 | 0.891903 |
Target: 5'- aCGUCGACACGGCUcaUGaaugGGUgcaCGgGCa -3' miRNA: 3'- -GUAGCUGUGCCGA--ACg---CCGa--GCgCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 200946 | 0.68 | 0.891903 |
Target: 5'- -cUUGGCACaguGGCUcaUGCGcGCUCGgGCg -3' miRNA: 3'- guAGCUGUG---CCGA--ACGC-CGAGCgCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 96121 | 0.68 | 0.885509 |
Target: 5'- --gCGACAUGGCgguucGCGGCcccCGUGCg -3' miRNA: 3'- guaGCUGUGCCGaa---CGCCGa--GCGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 292745 | 0.68 | 0.878917 |
Target: 5'- aUAUCGGCcccauuuuugACGGCUguUGCGuGCgauacgcaUCGCGCUg -3' miRNA: 3'- -GUAGCUG----------UGCCGA--ACGC-CG--------AGCGCGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 125502 | 0.68 | 0.865153 |
Target: 5'- uCAUCGACACGGaCUccGcCGGCUCuuGCc -3' miRNA: 3'- -GUAGCUGUGCC-GAa-C-GCCGAGcgCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 180144 | 0.69 | 0.846156 |
Target: 5'- gGUCGACAgcggagcggguuuccCGGacaGUGGCUCGCGUg -3' miRNA: 3'- gUAGCUGU---------------GCCgaaCGCCGAGCGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 128149 | 0.69 | 0.843128 |
Target: 5'- cCAUUGACACGGCgaccgaauauugUUGUGGgUCGCuCUc -3' miRNA: 3'- -GUAGCUGUGCCG------------AACGCCgAGCGcGA- -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 263167 | 0.69 | 0.843128 |
Target: 5'- aAUCGAacgaGCGGCgucguccGCGGCUUGUGUc -3' miRNA: 3'- gUAGCUg---UGCCGaa-----CGCCGAGCGCGa -5' |
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10300 | 3' | -58.1 | NC_002687.1 | + | 212381 | 0.69 | 0.835439 |
Target: 5'- gGUCGACGUGGCgagggggGCGGCcuuguUCGCGUc -3' miRNA: 3'- gUAGCUGUGCCGaa-----CGCCG-----AGCGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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