Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1076 | 3' | -49.1 | NC_000929.1 | + | 4404 | 0.66 | 0.924237 |
Target: 5'- uCCcgCGCGCCUGuaaccaguucUGCGCAucCGUu -3' miRNA: 3'- -GGuaGUGCGGACuga-------AUGCGUuuGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 5243 | 0.74 | 0.493429 |
Target: 5'- gCCAUUGCGUCUGGCgaUGCGUuuAACGUg -3' miRNA: 3'- -GGUAGUGCGGACUGa-AUGCGu-UUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 5904 | 0.66 | 0.925478 |
Target: 5'- gCAUCACGUCUGGaa-ACGgAAAUGa -3' miRNA: 3'- gGUAGUGCGGACUgaaUGCgUUUGCg -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 6703 | 0.67 | 0.90555 |
Target: 5'- aCCAUCccCGCgCUGGCaaaagaguUUGCGaCGGGCGUg -3' miRNA: 3'- -GGUAGu-GCG-GACUG--------AAUGC-GUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 8532 | 0.69 | 0.810121 |
Target: 5'- --uUCAgCGCCUGAC---CGCgAAACGCa -3' miRNA: 3'- gguAGU-GCGGACUGaauGCG-UUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 12344 | 0.72 | 0.635806 |
Target: 5'- uCCAUCACGCCUccugucaugcacgggGACgaggACGCuugacaccggcAACGCg -3' miRNA: 3'- -GGUAGUGCGGA---------------CUGaa--UGCGu----------UUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 12788 | 0.66 | 0.930906 |
Target: 5'- --cUCGCGCCgguggcugGACaUUGCGUggauuccAGACGCu -3' miRNA: 3'- gguAGUGCGGa-------CUG-AAUGCG-------UUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 13082 | 0.68 | 0.848545 |
Target: 5'- gUCGUaCugGCCUGACguggGCcgaaGCAGGcCGCa -3' miRNA: 3'- -GGUA-GugCGGACUGaa--UG----CGUUU-GCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 13710 | 0.73 | 0.561128 |
Target: 5'- gCCAUCACGCCaGGCUUuuucCGCuuccGGCGa -3' miRNA: 3'- -GGUAGUGCGGaCUGAAu---GCGu---UUGCg -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 13752 | 0.68 | 0.83742 |
Target: 5'- uCUGUCACGCgugCUGAUUgaagccgccaugACGCcAGCGCg -3' miRNA: 3'- -GGUAGUGCG---GACUGAa-----------UGCGuUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 13986 | 1.16 | 0.001014 |
Target: 5'- gCCAUCACGCCUGACUUACGCAAACGCg -3' miRNA: 3'- -GGUAGUGCGGACUGAAUGCGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 14656 | 0.67 | 0.89072 |
Target: 5'- -uGUgACGCCgaaccgGGCc-GCGCAGAUGCu -3' miRNA: 3'- ggUAgUGCGGa-----CUGaaUGCGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 15344 | 0.66 | 0.931493 |
Target: 5'- uCCAUCACgGCCUGcaucagcgugGCUUucuCGCGGuuCGUu -3' miRNA: 3'- -GGUAGUG-CGGAC----------UGAAu--GCGUUu-GCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 16256 | 0.7 | 0.757424 |
Target: 5'- uCCAUC-CGggUGGCUUugGCAAcgguaaagGCGCg -3' miRNA: 3'- -GGUAGuGCggACUGAAugCGUU--------UGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 18279 | 0.71 | 0.689542 |
Target: 5'- aUCAgCACGuCCUGGUUgagcGCGCGAACGCc -3' miRNA: 3'- -GGUaGUGC-GGACUGAa---UGCGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 18296 | 0.7 | 0.72395 |
Target: 5'- cUCGUCGCGgCUGGCUgguuuaACGCcgaugAAAUGCa -3' miRNA: 3'- -GGUAGUGCgGACUGAa-----UGCG-----UUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 18654 | 0.67 | 0.874697 |
Target: 5'- aCCGUCACcCCaaGAC--GCGCAAGCaGCu -3' miRNA: 3'- -GGUAGUGcGGa-CUGaaUGCGUUUG-CG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 21881 | 0.68 | 0.857533 |
Target: 5'- uCCAUgCugGCaCUGAUg---GCAGAUGCa -3' miRNA: 3'- -GGUA-GugCG-GACUGaaugCGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 22557 | 0.67 | 0.90555 |
Target: 5'- gUCAUUACGgCUGAC--AC-CAAACGUg -3' miRNA: 3'- -GGUAGUGCgGACUGaaUGcGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 24579 | 0.66 | 0.925478 |
Target: 5'- uCCGUCAUGCCgaaGcCUUuCGaCGAACGg -3' miRNA: 3'- -GGUAGUGCGGa--CuGAAuGC-GUUUGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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