Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1076 | 3' | -49.1 | NC_000929.1 | + | 25318 | 0.67 | 0.90555 |
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Target: 5'- gCCAUgACGUCUGAg--ACGCuguUGCa -3' miRNA: 3'- -GGUAgUGCGGACUgaaUGCGuuuGCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 27073 | 0.69 | 0.810121 |
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Target: 5'- aCGUCAUG-CUGGCagcgGCGCAGACa- -3' miRNA: 3'- gGUAGUGCgGACUGaa--UGCGUUUGcg -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 27995 | 0.66 | 0.919148 |
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Target: 5'- -gAUUGCGCgUGACcugUGCGCuccuuAugGCg -3' miRNA: 3'- ggUAGUGCGgACUGa--AUGCGu----UugCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 28837 | 0.76 | 0.409576 |
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Target: 5'- gCGUCguguGCGCCUGauGCUUGCGC--GCGCu -3' miRNA: 3'- gGUAG----UGCGGAC--UGAAUGCGuuUGCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 29614 | 0.67 | 0.898287 |
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Target: 5'- ---cCACGCCgGAU--ACGCugGAACGCg -3' miRNA: 3'- gguaGUGCGGaCUGaaUGCG--UUUGCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 31037 | 0.71 | 0.701093 |
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Target: 5'- -aAUCACGUCgcugaUGGCcUGCGCcAGCGCa -3' miRNA: 3'- ggUAGUGCGG-----ACUGaAUGCGuUUGCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 31273 | 0.69 | 0.789588 |
|
Target: 5'- uCCGUCA-GCCUGACgguaaaacCGUAcuGCGCa -3' miRNA: 3'- -GGUAGUgCGGACUGaau-----GCGUu-UGCG- -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 33408 | 0.67 | 0.882855 |
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Target: 5'- uCCGUCGCcauauucaGCCUGACa-GCGCGuuccAGCGg -3' miRNA: 3'- -GGUAGUG--------CGGACUGaaUGCGU----UUGCg -5' |
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| 1076 | 3' | -49.1 | NC_000929.1 | + | 36364 | 0.67 | 0.874697 |
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Target: 5'- --uUCgACGCCUGAUacacaACGCcuGCGCg -3' miRNA: 3'- gguAG-UGCGGACUGaa---UGCGuuUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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