Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1076 | 3' | -49.1 | NC_000929.1 | + | 22557 | 0.67 | 0.90555 |
Target: 5'- gUCAUUACGgCUGAC--AC-CAAACGUg -3' miRNA: 3'- -GGUAGUGCgGACUGaaUGcGUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 25318 | 0.67 | 0.90555 |
Target: 5'- gCCAUgACGUCUGAg--ACGCuguUGCa -3' miRNA: 3'- -GGUAgUGCGGACUgaaUGCGuuuGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 6703 | 0.67 | 0.90555 |
Target: 5'- aCCAUCccCGCgCUGGCaaaagaguUUGCGaCGGGCGUg -3' miRNA: 3'- -GGUAGu-GCG-GACUG--------AAUGC-GUUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 27995 | 0.66 | 0.919148 |
Target: 5'- -gAUUGCGCgUGACcugUGCGCuccuuAugGCg -3' miRNA: 3'- ggUAGUGCGgACUGa--AUGCGu----UugCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 4404 | 0.66 | 0.924237 |
Target: 5'- uCCcgCGCGCCUGuaaccaguucUGCGCAucCGUu -3' miRNA: 3'- -GGuaGUGCGGACuga-------AUGCGUuuGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 5904 | 0.66 | 0.925478 |
Target: 5'- gCAUCACGUCUGGaa-ACGgAAAUGa -3' miRNA: 3'- gGUAGUGCGGACUgaaUGCgUUUGCg -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 24579 | 0.66 | 0.925478 |
Target: 5'- uCCGUCAUGCCgaaGcCUUuCGaCGAACGg -3' miRNA: 3'- -GGUAGUGCGGa--CuGAAuGC-GUUUGCg -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 12788 | 0.66 | 0.930906 |
Target: 5'- --cUCGCGCCgguggcugGACaUUGCGUggauuccAGACGCu -3' miRNA: 3'- gguAGUGCGGa-------CUG-AAUGCG-------UUUGCG- -5' |
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1076 | 3' | -49.1 | NC_000929.1 | + | 15344 | 0.66 | 0.931493 |
Target: 5'- uCCAUCACgGCCUGcaucagcgugGCUUucuCGCGGuuCGUu -3' miRNA: 3'- -GGUAGUG-CGGAC----------UGAAu--GCGUUu-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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