miRNA: 3'- uUGGa---GGUUGUGUGGGUaGGUUAugu -5'
miRNA: 3'- uUGGAGGUu-GUGuGGGU---AGGU----UAUGU- -5'
miRNA: 3'- -UUGGA-----GGUUGUGUGGgUAGGUuaUGU- -5'
miRNA: 3'- uUGGAGGUUGUgUGGGUaGGUUaugu -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11181 | 5' | -50.5 | NC_002816.1 | + | 85128 | 0.66 | 0.981295 |
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Target: 5'- cACCguaaUCAACACACCCA-CCAAUc-- -3' miRNA: 3'- uUGGa---GGUUGUGUGGGUaGGUUAugu -5' |
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| 11181 | 5' | -50.5 | NC_002816.1 | + | 60613 | 0.66 | 0.983414 |
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Target: 5'- cACCUCCGucCGC-CCCAcccUCCAccucGUACAc -3' miRNA: 3'- uUGGAGGUu-GUGuGGGU---AGGU----UAUGU- -5' |
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| 11181 | 5' | -50.5 | NC_002816.1 | + | 59755 | 0.66 | 0.985347 |
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Target: 5'- cAACCUgcacaCCGAUACACCaAUCUAcgACAa -3' miRNA: 3'- -UUGGA-----GGUUGUGUGGgUAGGUuaUGU- -5' |
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| 11181 | 5' | -50.5 | NC_002816.1 | + | 28733 | 0.66 | 0.987103 |
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Target: 5'- uACCUCCAAUAaACCCA-CCGAc--- -3' miRNA: 3'- uUGGAGGUUGUgUGGGUaGGUUaugu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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