Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11283 | 3' | -53 | NC_003083.1 | + | 89504 | 0.67 | 0.920463 |
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Target: 5'- uGGCACCGUacGCGCACAGgguaacguUCAcguaugacaugacGGCCAg -3' miRNA: 3'- gUUGUGGCA--CGCGUGUU--------AGUa------------CCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 100625 | 0.68 | 0.868446 |
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Target: 5'- gCAACGgCGUGCGCACugaaCGcUGGCUu -3' miRNA: 3'- -GUUGUgGCACGCGUGuua-GU-ACCGGu -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 100752 | 0.7 | 0.780261 |
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Target: 5'- -cACGCCGuUGCGCACAugcgCGUGuCCAg -3' miRNA: 3'- guUGUGGC-ACGCGUGUua--GUACcGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 102138 | 0.78 | 0.342983 |
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Target: 5'- uGGCGCCGUGCGCGCGuuguacGUUAUGGgCGu -3' miRNA: 3'- gUUGUGGCACGCGUGU------UAGUACCgGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 102449 | 0.69 | 0.826774 |
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Target: 5'- cCAACGCCGUGUuaGCGCA---AUGGCg- -3' miRNA: 3'- -GUUGUGGCACG--CGUGUuagUACCGgu -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 104947 | 0.67 | 0.916894 |
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Target: 5'- aCAugACCGgcgGCGCGUAuguuuaaauuGUUGUGGCCGc -3' miRNA: 3'- -GUugUGGCa--CGCGUGU----------UAGUACCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 105049 | 0.68 | 0.868446 |
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Target: 5'- gGGCGUCGUGCGUugGAUguUGuGCCGg -3' miRNA: 3'- gUUGUGGCACGCGugUUAguAC-CGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 114504 | 0.66 | 0.943758 |
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Target: 5'- gCAAUACCGUGaGCaaGCAGUacCAUGaGCCGc -3' miRNA: 3'- -GUUGUGGCACgCG--UGUUA--GUAC-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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