Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11283 | 3' | -53 | NC_003083.1 | + | 87337 | 0.66 | 0.933798 |
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Target: 5'- -cGCACCGUGCGCugcCGGUCAaGuaCGu -3' miRNA: 3'- guUGUGGCACGCGu--GUUAGUaCcgGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 85367 | 0.66 | 0.933798 |
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Target: 5'- uCAAUugCuUGCGCGCcAUCAcgGGUCAa -3' miRNA: 3'- -GUUGugGcACGCGUGuUAGUa-CCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 114504 | 0.66 | 0.943758 |
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Target: 5'- gCAAUACCGUGaGCaaGCAGUacCAUGaGCCGc -3' miRNA: 3'- -GUUGUGGCACgCG--UGUUA--GUAC-CGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 83125 | 0.66 | 0.943758 |
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Target: 5'- aAAUAUUGUGCGCACAAUUcUGuuuguugguuGCCGg -3' miRNA: 3'- gUUGUGGCACGCGUGUUAGuAC----------CGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 9696 | 0.66 | 0.943758 |
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Target: 5'- aCGAUAgCGUGUGCAUAAUgaaccCAUGcaGCCAa -3' miRNA: 3'- -GUUGUgGCACGCGUGUUA-----GUAC--CGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 73688 | 0.66 | 0.943758 |
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Target: 5'- --uUAaaGUGCGCGauuGGUCAUGGCUAu -3' miRNA: 3'- guuGUggCACGCGUg--UUAGUACCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 58689 | 0.66 | 0.952685 |
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Target: 5'- uCAAC-CCGUGCgauacGCGCGcgCccGGCCu -3' miRNA: 3'- -GUUGuGGCACG-----CGUGUuaGuaCCGGu -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 63409 | 0.66 | 0.952685 |
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Target: 5'- gGACGgCGUGCGCG--GUCGUGGg-- -3' miRNA: 3'- gUUGUgGCACGCGUguUAGUACCggu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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