Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11283 | 3' | -53 | NC_003083.1 | + | 100752 | 0.7 | 0.780261 |
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Target: 5'- -cACGCCGuUGCGCACAugcgCGUGuCCAg -3' miRNA: 3'- guUGUGGC-ACGCGUGUua--GUACcGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 64493 | 0.7 | 0.760564 |
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Target: 5'- cCAACGCCGU-CGC-CAAcacUAUGGCCAa -3' miRNA: 3'- -GUUGUGGCAcGCGuGUUa--GUACCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 62798 | 0.71 | 0.750523 |
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Target: 5'- aAAUGaCGUGCGCACGcgCGUGGCg- -3' miRNA: 3'- gUUGUgGCACGCGUGUuaGUACCGgu -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 27951 | 0.72 | 0.698815 |
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Target: 5'- -cGCGUCGUGCGCGCucAAUUcgGGCCGc -3' miRNA: 3'- guUGUGGCACGCGUG--UUAGuaCCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 41688 | 0.73 | 0.592008 |
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Target: 5'- -cACGCagaGUGCGCACGcGUCAUGGUUAc -3' miRNA: 3'- guUGUGg--CACGCGUGU-UAGUACCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 102138 | 0.78 | 0.342983 |
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Target: 5'- uGGCGCCGUGCGCGCGuuguacGUUAUGGgCGu -3' miRNA: 3'- gUUGUGGCACGCGUGU------UAGUACCgGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 30526 | 0.79 | 0.327161 |
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Target: 5'- aCGACACCGccGCGCACA---AUGGCCAu -3' miRNA: 3'- -GUUGUGGCa-CGCGUGUuagUACCGGU- -5' |
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| 11283 | 3' | -53 | NC_003083.1 | + | 43443 | 1.09 | 0.003834 |
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Target: 5'- aCAACACCGUGCGCACAAUCAUGGCCAc -3' miRNA: 3'- -GUUGUGGCACGCGUGUUAGUACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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