Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11315 | 5' | -55.9 | NC_003084.1 | + | 9349 | 0.69 | 0.666937 |
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Target: 5'- cGGugCGCCCaGCuCGGCAgcgaCAAGUUGa -3' miRNA: 3'- uCCugGUGGG-CGuGCUGUa---GUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 10795 | 0.68 | 0.708497 |
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Target: 5'- cGGGaACCACCUcgagcaGCGCaACcgCGAGCUGa -3' miRNA: 3'- -UCC-UGGUGGG------CGUGcUGuaGUUCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 47783 | 0.68 | 0.708497 |
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Target: 5'- cAGGugUACUCuuucaGCACGGCGUUggGCa- -3' miRNA: 3'- -UCCugGUGGG-----CGUGCUGUAGuuCGac -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 101530 | 0.68 | 0.718741 |
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Target: 5'- aGGGACCGgUCGaACcGCAUCAAGCUc -3' miRNA: 3'- -UCCUGGUgGGCgUGcUGUAGUUCGAc -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 94939 | 0.68 | 0.732948 |
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Target: 5'- gAGGGCCACCaccggauccaccuugCGCugGACGauGAGCg- -3' miRNA: 3'- -UCCUGGUGG---------------GCGugCUGUagUUCGac -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 19912 | 0.68 | 0.748961 |
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Target: 5'- cGGGACCAUCguUGCAcCGAUuUCAaaGGCUGa -3' miRNA: 3'- -UCCUGGUGG--GCGU-GCUGuAGU--UCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 2108 | 0.67 | 0.75883 |
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Target: 5'- cAGccCCGCCCGCACaACAUUGgAGCUGu -3' miRNA: 3'- -UCcuGGUGGGCGUGcUGUAGU-UCGAC- -5' |
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| 11315 | 5' | -55.9 | NC_003084.1 | + | 65847 | 0.78 | 0.243084 |
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Target: 5'- aAGGugCACCCGCcCGACGgguuAGCUGg -3' miRNA: 3'- -UCCugGUGGGCGuGCUGUagu-UCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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