Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11319 | 5' | -49.9 | NC_003084.1 | + | 21839 | 0.68 | 0.973912 |
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Target: 5'- uUCCAUCGCCGUgu-ACUCGAUaucacCCGu -3' miRNA: 3'- -GGGUGGUGGCAaauUGGGCUAaa---GGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 21558 | 0.69 | 0.967924 |
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Target: 5'- aCCACCACCGcgua--CCGG--UCCGg -3' miRNA: 3'- gGGUGGUGGCaaauugGGCUaaAGGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 19708 | 0.67 | 0.988578 |
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Target: 5'- aCC-UCGCCGggugcUAGCCCGuUUUCCa -3' miRNA: 3'- gGGuGGUGGCaa---AUUGGGCuAAAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 18584 | 0.67 | 0.987011 |
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Target: 5'- aCCACCACCGUgcgGGCCauc--UCUGc -3' miRNA: 3'- gGGUGGUGGCAaa-UUGGgcuaaAGGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 18217 | 0.66 | 0.993433 |
|
Target: 5'- aCCCaacGCCACCGgcu-GCaCCGAauugUCCa -3' miRNA: 3'- -GGG---UGGUGGCaaauUG-GGCUaa--AGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 16235 | 0.74 | 0.809658 |
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Target: 5'- gCCCuCCACCGUUguaucACCCGG--UCCa -3' miRNA: 3'- -GGGuGGUGGCAAau---UGGGCUaaAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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