Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11319 | 5' | -49.9 | NC_003084.1 | + | 48862 | 0.7 | 0.94428 |
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Target: 5'- gCCACCACCGgUUUAACgCG-UUUCg- -3' miRNA: 3'- gGGUGGUGGC-AAAUUGgGCuAAAGgc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 25467 | 0.7 | 0.939458 |
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Target: 5'- gCCCGCCACCGUgucgGGCaucguCGGUagggCCGa -3' miRNA: 3'- -GGGUGGUGGCAaa--UUGg----GCUAaa--GGC- -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 62058 | 0.7 | 0.927375 |
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Target: 5'- aCCACCACCGUUgcuaccacuacauguGCCCaGUUUCa- -3' miRNA: 3'- gGGUGGUGGCAAau-------------UGGGcUAAAGgc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 90262 | 0.71 | 0.915131 |
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Target: 5'- gCCCACCAgCGguguaacggugGACCCGAaugcgUUUCCc -3' miRNA: 3'- -GGGUGGUgGCaaa--------UUGGGCU-----AAAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 16235 | 0.74 | 0.809658 |
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Target: 5'- gCCCuCCACCGUUguaucACCCGG--UCCa -3' miRNA: 3'- -GGGuGGUGGCAAau---UGGGCUaaAGGc -5' |
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| 11319 | 5' | -49.9 | NC_003084.1 | + | 24482 | 1.12 | 0.006672 |
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Target: 5'- cCCCACCACCGUUUAACCCGAUUUCCGu -3' miRNA: 3'- -GGGUGGUGGCAAAUUGGGCUAAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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